Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19573 | 3' | -58.9 | NC_004686.1 | + | 3977 | 0.66 | 0.546794 |
Target: 5'- aGGAGGAUCCGcaggccgcGGAUacaugcggCGCAcCCGGCc -3' miRNA: 3'- aCCUCUUAGGCc-------CCUA--------GCGU-GGCCGc -5' |
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19573 | 3' | -58.9 | NC_004686.1 | + | 49838 | 0.66 | 0.545755 |
Target: 5'- cGGAGcugGAUCgGGGcAUCGCcauaacccgcgugGCCGGCu -3' miRNA: 3'- aCCUC---UUAGgCCCcUAGCG-------------UGGCCGc -5' |
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19573 | 3' | -58.9 | NC_004686.1 | + | 49119 | 0.66 | 0.536427 |
Target: 5'- cGGGGGugugucauuccAUCC-GGGAUUGCACgUGGCu -3' miRNA: 3'- aCCUCU-----------UAGGcCCCUAGCGUG-GCCGc -5' |
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19573 | 3' | -58.9 | NC_004686.1 | + | 11593 | 0.66 | 0.536427 |
Target: 5'- cUGGuAGGcguagucgAUCgCGGGGAUCGCGCCa--- -3' miRNA: 3'- -ACC-UCU--------UAG-GCCCCUAGCGUGGccgc -5' |
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19573 | 3' | -58.9 | NC_004686.1 | + | 38501 | 0.66 | 0.536427 |
Target: 5'- cGGcGAGUCCGGGGccgaugaUGCcCgCGGCGa -3' miRNA: 3'- aCCuCUUAGGCCCCua-----GCGuG-GCCGC- -5' |
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19573 | 3' | -58.9 | NC_004686.1 | + | 10995 | 0.66 | 0.52613 |
Target: 5'- uUGGaAGcGUCCacguuucgguGGGGGUCGUGCucaCGGCGg -3' miRNA: 3'- -ACC-UCuUAGG----------CCCCUAGCGUG---GCCGC- -5' |
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19573 | 3' | -58.9 | NC_004686.1 | + | 26522 | 0.67 | 0.475879 |
Target: 5'- aGGcaaguGGGAUCCGGGGAaccUCGaCAUgucgugguuccgCGGCGa -3' miRNA: 3'- aCC-----UCUUAGGCCCCU---AGC-GUG------------GCCGC- -5' |
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19573 | 3' | -58.9 | NC_004686.1 | + | 3825 | 0.67 | 0.466113 |
Target: 5'- -cGAGGAUCuUGGGGccuaCGCGCUGGCc -3' miRNA: 3'- acCUCUUAG-GCCCCua--GCGUGGCCGc -5' |
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19573 | 3' | -58.9 | NC_004686.1 | + | 45835 | 0.67 | 0.456451 |
Target: 5'- gUGGuGAAUUCGGcGGugaCGgGCCGGCc -3' miRNA: 3'- -ACCuCUUAGGCC-CCua-GCgUGGCCGc -5' |
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19573 | 3' | -58.9 | NC_004686.1 | + | 46957 | 0.68 | 0.428133 |
Target: 5'- aUGGGGGgugagucguacgGUCCGGGcGGUgGCuCgGGCGg -3' miRNA: 3'- -ACCUCU------------UAGGCCC-CUAgCGuGgCCGC- -5' |
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19573 | 3' | -58.9 | NC_004686.1 | + | 11135 | 0.68 | 0.418926 |
Target: 5'- gUGGGcAGUCCGGGGcauaugCGUugCGuGCGg -3' miRNA: 3'- -ACCUcUUAGGCCCCua----GCGugGC-CGC- -5' |
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19573 | 3' | -58.9 | NC_004686.1 | + | 14021 | 0.68 | 0.418926 |
Target: 5'- cGGAGAAUCgaaCGGcaccucGGuUUGCGCCGGCc -3' miRNA: 3'- aCCUCUUAG---GCC------CCuAGCGUGGCCGc -5' |
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19573 | 3' | -58.9 | NC_004686.1 | + | 17022 | 0.7 | 0.341761 |
Target: 5'- aUGGGugcGGA-CUGGuGGAUCGCucCCGGCGg -3' miRNA: 3'- -ACCU---CUUaGGCC-CCUAGCGu-GGCCGC- -5' |
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19573 | 3' | -58.9 | NC_004686.1 | + | 37332 | 0.7 | 0.333063 |
Target: 5'- uUGGAGggUCCcggcgcgggagcgGGGGAaccaugccCGCGCCGGg- -3' miRNA: 3'- -ACCUCuuAGG-------------CCCCUa-------GCGUGGCCgc -5' |
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19573 | 3' | -58.9 | NC_004686.1 | + | 9875 | 0.7 | 0.303544 |
Target: 5'- gUGGGGAcgCCGGcGGcgacCGCAgUGGCGg -3' miRNA: 3'- -ACCUCUuaGGCC-CCua--GCGUgGCCGC- -5' |
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19573 | 3' | -58.9 | NC_004686.1 | + | 8779 | 0.71 | 0.274725 |
Target: 5'- gUGGAGAAcugcaccUCCGcGGaGAUCGCGCaGGCc -3' miRNA: 3'- -ACCUCUU-------AGGC-CC-CUAGCGUGgCCGc -5' |
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19573 | 3' | -58.9 | NC_004686.1 | + | 41211 | 0.72 | 0.243205 |
Target: 5'- cGGAGaAGUUgGGGGAggacgcccCGUACUGGCGa -3' miRNA: 3'- aCCUC-UUAGgCCCCUa-------GCGUGGCCGC- -5' |
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19573 | 3' | -58.9 | NC_004686.1 | + | 27722 | 0.74 | 0.169468 |
Target: 5'- cGGuGAugAUCCGGGcGAUCGCcgucugaucccgACCGGCa -3' miRNA: 3'- aCCuCU--UAGGCCC-CUAGCG------------UGGCCGc -5' |
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19573 | 3' | -58.9 | NC_004686.1 | + | 1976 | 0.78 | 0.095808 |
Target: 5'- cGGucagcAGGAUCuugaacugcugugCGGGGGUCGCGCCGGCa -3' miRNA: 3'- aCC-----UCUUAG-------------GCCCCUAGCGUGGCCGc -5' |
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19573 | 3' | -58.9 | NC_004686.1 | + | 11257 | 1.08 | 0.000632 |
Target: 5'- uUGGAGAAUCCGGGGAUCGCACCGGCGc -3' miRNA: 3'- -ACCUCUUAGGCCCCUAGCGUGGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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