Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19573 | 5' | -56.3 | NC_004686.1 | + | 39147 | 0.66 | 0.679733 |
Target: 5'- gGCACGUAuGCGCAgaccugcucgaaugaGUGCCgaCGGGCg -3' miRNA: 3'- gCGUGCGU-UGCGUa--------------UACGGg-GCCUGa -5' |
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19573 | 5' | -56.3 | NC_004686.1 | + | 28111 | 0.66 | 0.678646 |
Target: 5'- uCGCugGCggUGC---UGCUCUGGugUc -3' miRNA: 3'- -GCGugCGuuGCGuauACGGGGCCugA- -5' |
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19573 | 5' | -56.3 | NC_004686.1 | + | 14591 | 0.66 | 0.656848 |
Target: 5'- gGUugGCGccGCGCcacgGCUCCGGAUc -3' miRNA: 3'- gCGugCGU--UGCGuauaCGGGGCCUGa -5' |
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19573 | 5' | -56.3 | NC_004686.1 | + | 15673 | 0.66 | 0.656848 |
Target: 5'- uGCcCGCGAUGCG-GUGgCCCGG-CUg -3' miRNA: 3'- gCGuGCGUUGCGUaUACgGGGCCuGA- -5' |
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19573 | 5' | -56.3 | NC_004686.1 | + | 11769 | 0.67 | 0.613059 |
Target: 5'- cCGUugGUucCGaCAUucaAUGCUCCGGGCUu -3' miRNA: 3'- -GCGugCGuuGC-GUA---UACGGGGCCUGA- -5' |
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19573 | 5' | -56.3 | NC_004686.1 | + | 18284 | 0.67 | 0.613059 |
Target: 5'- aGCACgGCAGCGgGgcgaGCcuCCCGGACUu -3' miRNA: 3'- gCGUG-CGUUGCgUaua-CG--GGGCCUGA- -5' |
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19573 | 5' | -56.3 | NC_004686.1 | + | 26632 | 0.67 | 0.602129 |
Target: 5'- cCGgGC-CGACGC---UGCCuCCGGACUa -3' miRNA: 3'- -GCgUGcGUUGCGuauACGG-GGCCUGA- -5' |
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19573 | 5' | -56.3 | NC_004686.1 | + | 8573 | 0.67 | 0.602129 |
Target: 5'- --gGCGCAACGCGgcuuugAUGUCaCGGACa -3' miRNA: 3'- gcgUGCGUUGCGUa-----UACGGgGCCUGa -5' |
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19573 | 5' | -56.3 | NC_004686.1 | + | 39942 | 0.68 | 0.526796 |
Target: 5'- aGCACGCGugGCGUgaccaagcguuGUGCuCCCaGGuCg -3' miRNA: 3'- gCGUGCGUugCGUA-----------UACG-GGG-CCuGa -5' |
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19573 | 5' | -56.3 | NC_004686.1 | + | 12972 | 0.69 | 0.485322 |
Target: 5'- gGCGCGCcGCGCAaagcagaaGUCCCGGAa- -3' miRNA: 3'- gCGUGCGuUGCGUaua-----CGGGGCCUga -5' |
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19573 | 5' | -56.3 | NC_004686.1 | + | 37493 | 0.69 | 0.485322 |
Target: 5'- aGCGCGCuucggcgauACGCAUcgucaaGUcGCCCuCGGACa -3' miRNA: 3'- gCGUGCGu--------UGCGUA------UA-CGGG-GCCUGa -5' |
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19573 | 5' | -56.3 | NC_004686.1 | + | 21840 | 0.7 | 0.426239 |
Target: 5'- gGCGCG-GGCGCGggcggcgGCUCCGGACg -3' miRNA: 3'- gCGUGCgUUGCGUaua----CGGGGCCUGa -5' |
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19573 | 5' | -56.3 | NC_004686.1 | + | 10596 | 0.7 | 0.426239 |
Target: 5'- cCGcCGCGCAGguCGCcgAUGCUuuGGGCg -3' miRNA: 3'- -GC-GUGCGUU--GCGuaUACGGggCCUGa -5' |
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19573 | 5' | -56.3 | NC_004686.1 | + | 11601 | 0.71 | 0.354537 |
Target: 5'- uGCGCGCAugGCAggucuucCUCCGGGCc -3' miRNA: 3'- gCGUGCGUugCGUauac---GGGGCCUGa -5' |
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19573 | 5' | -56.3 | NC_004686.1 | + | 42926 | 0.71 | 0.346194 |
Target: 5'- uGCACacCAcCGCAg--GCCCCGGGCUc -3' miRNA: 3'- gCGUGc-GUuGCGUauaCGGGGCCUGA- -5' |
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19573 | 5' | -56.3 | NC_004686.1 | + | 45945 | 0.72 | 0.30661 |
Target: 5'- -aCACGCAAUGCcUAUGCCCaGGAUUc -3' miRNA: 3'- gcGUGCGUUGCGuAUACGGGgCCUGA- -5' |
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19573 | 5' | -56.3 | NC_004686.1 | + | 10757 | 0.74 | 0.25065 |
Target: 5'- gGCAccccCGCGGCGCAagccGCCCUGGACg -3' miRNA: 3'- gCGU----GCGUUGCGUaua-CGGGGCCUGa -5' |
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19573 | 5' | -56.3 | NC_004686.1 | + | 38417 | 0.74 | 0.225974 |
Target: 5'- cCGCGgGCAu--CAUcgGCCCCGGACUc -3' miRNA: 3'- -GCGUgCGUugcGUAuaCGGGGCCUGA- -5' |
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19573 | 5' | -56.3 | NC_004686.1 | + | 49683 | 0.79 | 0.10531 |
Target: 5'- aGCACGCAGCGCAUGUGgCCgaGGAa- -3' miRNA: 3'- gCGUGCGUUGCGUAUACgGGg-CCUga -5' |
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19573 | 5' | -56.3 | NC_004686.1 | + | 11223 | 1.08 | 0.000868 |
Target: 5'- cCGCACGCAACGCAUAUGCCCCGGACUg -3' miRNA: 3'- -GCGUGCGUUGCGUAUACGGGGCCUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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