miRNA: 3'- cAC-GUGCguCu--CCACA--AGUAGCAAGa -5'
miRNA: 3'- -CACGUGCGUCUCCacaaGUaGCaaga -5'
miRNA: 3'- caCGugCGUCUCCACAAguA-GCAAGa -5'
miRNA: 3'- caCGugCGUCUCCAcaa--GUAGCAAGa -5'
miRNA: 3'- -CACGUGCGUCUCCACAAGUAGCAAGA- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19576 | 5' | -51.3 | NC_004686.1 | + | 44117 | 0.66 | 0.897732 |
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Target: 5'- uUGuCACGguGcguGGUGUuuUCGUCGUUUg -3' miRNA: 3'- cAC-GUGCguCu--CCACA--AGUAGCAAGa -5' |
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| 19576 | 5' | -51.3 | NC_004686.1 | + | 19601 | 0.66 | 0.874789 |
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Target: 5'- uGUGCGCGCAGAGGgaggCAcCGa--- -3' miRNA: 3'- -CACGUGCGUCUCCacaaGUaGCaaga -5' |
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| 19576 | 5' | -51.3 | NC_004686.1 | + | 23060 | 0.72 | 0.582132 |
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Target: 5'- -cGUugGCGGAGGUGUUguUgUGUUCg -3' miRNA: 3'- caCGugCGUCUCCACAAguA-GCAAGa -5' |
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| 19576 | 5' | -51.3 | NC_004686.1 | + | 1471 | 0.74 | 0.483272 |
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Target: 5'- -aGCugGCGGAGGUcaaucCGUCGUUCg -3' miRNA: 3'- caCGugCGUCUCCAcaa--GUAGCAAGa -5' |
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| 19576 | 5' | -51.3 | NC_004686.1 | + | 12930 | 1.09 | 0.002345 |
|
Target: 5'- cGUGCACGCAGAGGUGUUCAUCGUUCUg -3' miRNA: 3'- -CACGUGCGUCUCCACAAGUAGCAAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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