Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19580 | 5' | -55.3 | NC_004686.1 | + | 10526 | 0.66 | 0.708659 |
Target: 5'- aGUGCGCaacucGUUGGCGgUGUCgUGGu -3' miRNA: 3'- -CAUGCGgaa--CAACCGUgGCAGgACCu -5' |
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19580 | 5' | -55.3 | NC_004686.1 | + | 29242 | 0.67 | 0.653958 |
Target: 5'- cGUGauCGCgUUGUagaaUGGguCCGUgCCUGGAu -3' miRNA: 3'- -CAU--GCGgAACA----ACCguGGCA-GGACCU- -5' |
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19580 | 5' | -55.3 | NC_004686.1 | + | 22101 | 0.73 | 0.317483 |
Target: 5'- -cGCGCCgccGUcGGCGCCGUCCUugccgucaccGGAa -3' miRNA: 3'- caUGCGGaa-CAaCCGUGGCAGGA----------CCU- -5' |
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19580 | 5' | -55.3 | NC_004686.1 | + | 15116 | 1.09 | 0.000994 |
Target: 5'- uGUACGCCUUGUUGGCACCGUCCUGGAa -3' miRNA: 3'- -CAUGCGGAACAACCGUGGCAGGACCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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