Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19582 | 5' | -61.3 | NC_004686.1 | + | 9788 | 0.7 | 0.234294 |
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Target: 5'- uGCCGc-CACUGCGGUCgCCGCcGGCGu -3' miRNA: 3'- cCGGCucGUGGCGUCAG-GGUGuCCGC- -5' |
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| 19582 | 5' | -61.3 | NC_004686.1 | + | 8816 | 0.71 | 0.196222 |
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Target: 5'- aGGCCGAGUGggaucggguguCUGCGGUUCCGCuGGgGg -3' miRNA: 3'- -CCGGCUCGU-----------GGCGUCAGGGUGuCCgC- -5' |
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| 19582 | 5' | -61.3 | NC_004686.1 | + | 40695 | 0.72 | 0.167564 |
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Target: 5'- uGGUCGAacguggcggcgucGCaACCGguGUCCCaACGGGCa -3' miRNA: 3'- -CCGGCU-------------CG-UGGCguCAGGG-UGUCCGc -5' |
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| 19582 | 5' | -61.3 | NC_004686.1 | + | 17246 | 0.78 | 0.067063 |
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Target: 5'- uGGCCGcGCAUCGCGGcuUCCCACuccgacugaGGGCGg -3' miRNA: 3'- -CCGGCuCGUGGCGUC--AGGGUG---------UCCGC- -5' |
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| 19582 | 5' | -61.3 | NC_004686.1 | + | 15617 | 1.09 | 0.000267 |
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Target: 5'- gGGCCGAGCACCGCAGUCCCACAGGCGu -3' miRNA: 3'- -CCGGCUCGUGGCGUCAGGGUGUCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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