Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19584 | 5' | -53.9 | NC_004686.1 | + | 47132 | 0.66 | 0.796365 |
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Target: 5'- cGGCUGUcCCUC-GCGgGUGAgcuucucaacCUGGa -3' miRNA: 3'- uCCGACA-GGAGuCGC-CACUaua-------GACC- -5' |
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| 19584 | 5' | -53.9 | NC_004686.1 | + | 6140 | 0.66 | 0.788473 |
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Target: 5'- -aGCUGaUCCUgAucggcGCGGUGAUGcgCUGGu -3' miRNA: 3'- ucCGAC-AGGAgU-----CGCCACUAUa-GACC- -5' |
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| 19584 | 5' | -53.9 | NC_004686.1 | + | 4113 | 0.66 | 0.77846 |
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Target: 5'- gAGGCUGUCC-CGGC-GUGGggugGUCa-- -3' miRNA: 3'- -UCCGACAGGaGUCGcCACUa---UAGacc -5' |
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| 19584 | 5' | -53.9 | NC_004686.1 | + | 52199 | 0.69 | 0.594064 |
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Target: 5'- cGGUgaUGUCCUCAaccagGGUGGUGUCUuGGg -3' miRNA: 3'- uCCG--ACAGGAGUcg---CCACUAUAGA-CC- -5' |
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| 19584 | 5' | -53.9 | NC_004686.1 | + | 30854 | 0.71 | 0.486437 |
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Target: 5'- uGG-UGUCuCUCGGUGGUGAUGagUGGu -3' miRNA: 3'- uCCgACAG-GAGUCGCCACUAUagACC- -5' |
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| 19584 | 5' | -53.9 | NC_004686.1 | + | 10299 | 0.75 | 0.305154 |
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Target: 5'- cAGGUUGUCCagGGCGGUGGUGag-GGu -3' miRNA: 3'- -UCCGACAGGagUCGCCACUAUagaCC- -5' |
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| 19584 | 5' | -53.9 | NC_004686.1 | + | 16666 | 1.1 | 0.001131 |
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Target: 5'- cAGGCUGUCCUCAGCGGUGAUAUCUGGu -3' miRNA: 3'- -UCCGACAGGAGUCGCCACUAUAGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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