Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19585 | 3' | -52.2 | NC_004686.1 | + | 30693 | 0.66 | 0.847358 |
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Target: 5'- gGGCGcUGAugUGUGGUGGUUGUGguucCCAa -3' miRNA: 3'- -UCGCaACUcaACACCGCCAGUAU----GGU- -5' |
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| 19585 | 3' | -52.2 | NC_004686.1 | + | 27899 | 0.67 | 0.797518 |
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Target: 5'- gAGCGUccacacccgaacgaUGAGacaccgcgUGUGGaCGGUCAU-CCAa -3' miRNA: 3'- -UCGCA--------------ACUCa-------ACACC-GCCAGUAuGGU- -5' |
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| 19585 | 3' | -52.2 | NC_004686.1 | + | 54313 | 0.67 | 0.790516 |
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Target: 5'- aGGCGUUGGGgcUGUGuuccGCGG-CGUACUg -3' miRNA: 3'- -UCGCAACUCa-ACAC----CGCCaGUAUGGu -5' |
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| 19585 | 3' | -52.2 | NC_004686.1 | + | 42691 | 0.71 | 0.581765 |
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Target: 5'- uGUGUUGAcgggGUUGUGGCGGgaugCGaagGCCGg -3' miRNA: 3'- uCGCAACU----CAACACCGCCa---GUa--UGGU- -5' |
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| 19585 | 3' | -52.2 | NC_004686.1 | + | 13630 | 0.73 | 0.452739 |
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Target: 5'- cAGauaGUUGGuGUUGuUGGCGGUgCGUGCCAu -3' miRNA: 3'- -UCg--CAACU-CAAC-ACCGCCA-GUAUGGU- -5' |
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| 19585 | 3' | -52.2 | NC_004686.1 | + | 16981 | 0.93 | 0.024358 |
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Target: 5'- cAGCGUUGAGUUugGGCGGUCAUACCAg -3' miRNA: 3'- -UCGCAACUCAAcaCCGCCAGUAUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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