Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19588 | 3' | -52.1 | NC_004686.1 | + | 8772 | 0.66 | 0.886673 |
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Target: 5'- --cUCGGUGGuggaGAACugCACCUCcgCGGa -3' miRNA: 3'- gaaAGUCACC----CUUG--GUGGAGuaGCCa -5' |
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| 19588 | 3' | -52.1 | NC_004686.1 | + | 11117 | 0.66 | 0.871023 |
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Target: 5'- -cUUCGGgccGGGGCCACCg-AUCGGa -3' miRNA: 3'- gaAAGUCac-CCUUGGUGGagUAGCCa -5' |
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| 19588 | 3' | -52.1 | NC_004686.1 | + | 11009 | 0.67 | 0.818109 |
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Target: 5'- gUUUCGGUGGGggUCgugCUCAcggCGGUc -3' miRNA: 3'- gAAAGUCACCCuuGGug-GAGUa--GCCA- -5' |
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| 19588 | 3' | -52.1 | NC_004686.1 | + | 9057 | 0.69 | 0.70386 |
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Target: 5'- -gUUCGGUGGGAGCCAggcgaUCAggucgCGGa -3' miRNA: 3'- gaAAGUCACCCUUGGUgg---AGUa----GCCa -5' |
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| 19588 | 3' | -52.1 | NC_004686.1 | + | 52013 | 0.73 | 0.482935 |
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Target: 5'- ---gCAGUGGGAACCacgccccaACCUCGacgcaUCGGUc -3' miRNA: 3'- gaaaGUCACCCUUGG--------UGGAGU-----AGCCA- -5' |
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| 19588 | 3' | -52.1 | NC_004686.1 | + | 19892 | 1.09 | 0.002147 |
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Target: 5'- aCUUUCAGUGGGAACCACCUCAUCGGUg -3' miRNA: 3'- -GAAAGUCACCCUUGGUGGAGUAGCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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