Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19589 | 5' | -58 | NC_004686.1 | + | 22697 | 0.66 | 0.57394 |
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Target: 5'- cGGUGauggcgauCUGCCCG-GCGGuGggGGUGAc- -3' miRNA: 3'- -CCAU--------GACGGGCaCGCC-CgaCCACUac -5' |
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| 19589 | 5' | -58 | NC_004686.1 | + | 23736 | 0.66 | 0.57394 |
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Target: 5'- cGUGgUGCCCGUcaGGGUgagGGUGAc- -3' miRNA: 3'- cCAUgACGGGCAcgCCCGa--CCACUac -5' |
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| 19589 | 5' | -58 | NC_004686.1 | + | 46079 | 0.68 | 0.470582 |
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Target: 5'- aGUACgaagacgGCCCGgGCGGuGCUGGUa--- -3' miRNA: 3'- cCAUGa------CGGGCaCGCC-CGACCAcuac -5' |
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| 19589 | 5' | -58 | NC_004686.1 | + | 53045 | 0.68 | 0.460743 |
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Target: 5'- -aUGCUGCCCGUguagagGCGGGUUucgucuuugGGUGGg- -3' miRNA: 3'- ccAUGACGGGCA------CGCCCGA---------CCACUac -5' |
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| 19589 | 5' | -58 | NC_004686.1 | + | 25121 | 0.71 | 0.312553 |
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Target: 5'- uGUGCUGCUCGUGCuGGGCgaugagGGcGAg- -3' miRNA: 3'- cCAUGACGGGCACG-CCCGa-----CCaCUac -5' |
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| 19589 | 5' | -58 | NC_004686.1 | + | 20005 | 1.1 | 0.00049 |
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Target: 5'- aGGUACUGCCCGUGCGGGCUGGUGAUGu -3' miRNA: 3'- -CCAUGACGGGCACGCCCGACCACUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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