Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19590 | 5' | -56.8 | NC_004686.1 | + | 12351 | 0.66 | 0.653533 |
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Target: 5'- -cCGgCGGCCACGACGa--CCGgugagCAGa -3' miRNA: 3'- caGCgGCCGGUGCUGCaaaGGCa----GUU- -5' |
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| 19590 | 5' | -56.8 | NC_004686.1 | + | 10360 | 0.68 | 0.51768 |
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Target: 5'- uGUCGCCGGCCuCGcuggugguauuggcaAUGUgcuuggagCCGUCAAc -3' miRNA: 3'- -CAGCGGCCGGuGC---------------UGCAaa------GGCAGUU- -5' |
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| 19590 | 5' | -56.8 | NC_004686.1 | + | 5515 | 0.69 | 0.470626 |
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Target: 5'- uUCGCCGGCCugaacGCGGCGgugucggacaCCGUCc- -3' miRNA: 3'- cAGCGGCCGG-----UGCUGCaaa-------GGCAGuu -5' |
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| 19590 | 5' | -56.8 | NC_004686.1 | + | 51850 | 0.69 | 0.452809 |
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Target: 5'- uUUGCgGGCUucgACGAUGUUgCCGUCAGc -3' miRNA: 3'- cAGCGgCCGG---UGCUGCAAaGGCAGUU- -5' |
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| 19590 | 5' | -56.8 | NC_004686.1 | + | 34733 | 0.69 | 0.443071 |
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Target: 5'- uUCGCCGGCUACGGCaa--CCG-CAAg -3' miRNA: 3'- cAGCGGCCGGUGCUGcaaaGGCaGUU- -5' |
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| 19590 | 5' | -56.8 | NC_004686.1 | + | 14711 | 0.7 | 0.40351 |
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Target: 5'- aGUCGUCGGCgaacucgGCGACGgugcgggccagUCCGUCAAa -3' miRNA: 3'- -CAGCGGCCGg------UGCUGCaa---------AGGCAGUU- -5' |
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| 19590 | 5' | -56.8 | NC_004686.1 | + | 24476 | 0.7 | 0.396231 |
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Target: 5'- cGUCGUCGGUCAugauuuucuUGACGgugCCGUCAc -3' miRNA: 3'- -CAGCGGCCGGU---------GCUGCaaaGGCAGUu -5' |
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| 19590 | 5' | -56.8 | NC_004686.1 | + | 42826 | 0.74 | 0.212954 |
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Target: 5'- aGUCGCCGGuUCACGGgGUUUCCcccggaaGUCAAg -3' miRNA: 3'- -CAGCGGCC-GGUGCUgCAAAGG-------CAGUU- -5' |
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| 19590 | 5' | -56.8 | NC_004686.1 | + | 20500 | 1.06 | 0.001216 |
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Target: 5'- cGUCGCCGGCCACGACGUUUCCGUCAAc -3' miRNA: 3'- -CAGCGGCCGGUGCUGCAAAGGCAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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