Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19594 | 5' | -64.8 | NC_004686.1 | + | 35054 | 0.7 | 0.124173 |
Target: 5'- uGGCCGUCACCgagGCUGgCGUcuggUCGCUGAUg -3' miRNA: 3'- -UCGGCAGUGG---CGACgGCG----GGCGGCUA- -5' |
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19594 | 5' | -64.8 | NC_004686.1 | + | 21917 | 0.71 | 0.114654 |
Target: 5'- gAGCCGcCGcCCGC-GcCCGCgCCGCCGAg -3' miRNA: 3'- -UCGGCaGU-GGCGaC-GGCG-GGCGGCUa -5' |
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19594 | 5' | -64.8 | NC_004686.1 | + | 48922 | 0.72 | 0.10029 |
Target: 5'- uGGCCGccgcccuucUCGCCGCUGCUGUUguaGCCGAg -3' miRNA: 3'- -UCGGC---------AGUGGCGACGGCGGg--CGGCUa -5' |
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19594 | 5' | -64.8 | NC_004686.1 | + | 39044 | 0.72 | 0.092505 |
Target: 5'- uGGCCGgcuUCACCGgUGCCaGCUCGCCa-- -3' miRNA: 3'- -UCGGC---AGUGGCgACGG-CGGGCGGcua -5' |
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19594 | 5' | -64.8 | NC_004686.1 | + | 22387 | 0.74 | 0.070498 |
Target: 5'- gGGCCGUCGCUgguuccguuGUUGCCG-CCGCUGAUg -3' miRNA: 3'- -UCGGCAGUGG---------CGACGGCgGGCGGCUA- -5' |
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19594 | 5' | -64.8 | NC_004686.1 | + | 22270 | 0.75 | 0.052114 |
Target: 5'- uGCUGcCGCCGUUGCCGCcguugCCGCCGGa -3' miRNA: 3'- uCGGCaGUGGCGACGGCG-----GGCGGCUa -5' |
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19594 | 5' | -64.8 | NC_004686.1 | + | 22435 | 1.04 | 0.000259 |
Target: 5'- gAGCCGUCACCGCUGCCGCCCGCCGAUc -3' miRNA: 3'- -UCGGCAGUGGCGACGGCGGGCGGCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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