Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19596 | 3' | -58.5 | NC_004686.1 | + | 47210 | 0.66 | 0.525877 |
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Target: 5'- -aGGUccucGGAUGGGCUGGCCUcggaaaCCCUg -3' miRNA: 3'- caCCGa---CUUGCCUGACCGGAac----GGGA- -5' |
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| 19596 | 3' | -58.5 | NC_004686.1 | + | 48776 | 0.67 | 0.425747 |
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Target: 5'- -gGGcCUGAACGGccGCUGGCCccGUCUUc -3' miRNA: 3'- caCC-GACUUGCC--UGACCGGaaCGGGA- -5' |
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| 19596 | 3' | -58.5 | NC_004686.1 | + | 39576 | 0.67 | 0.425747 |
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Target: 5'- gGUGGUgGAGCGGAUcgUGGCgUUGUCg- -3' miRNA: 3'- -CACCGaCUUGCCUG--ACCGgAACGGga -5' |
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| 19596 | 3' | -58.5 | NC_004686.1 | + | 37036 | 0.67 | 0.425747 |
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Target: 5'- gGUGGCUGggUGGAUccgUGGUUggcGCgCCUg -3' miRNA: 3'- -CACCGACuuGCCUG---ACCGGaa-CG-GGA- -5' |
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| 19596 | 3' | -58.5 | NC_004686.1 | + | 48908 | 0.68 | 0.407098 |
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Target: 5'- -cGGCgc-ACGGAauCUGGCCgccGCCCUu -3' miRNA: 3'- caCCGacuUGCCU--GACCGGaa-CGGGA- -5' |
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| 19596 | 3' | -58.5 | NC_004686.1 | + | 9263 | 0.69 | 0.36279 |
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Target: 5'- gGUGGCU-AACGGAacugaaCUGGCCgucGCCUa -3' miRNA: 3'- -CACCGAcUUGCCU------GACCGGaa-CGGGa -5' |
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| 19596 | 3' | -58.5 | NC_004686.1 | + | 8396 | 0.69 | 0.32116 |
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Target: 5'- uUGGCUGGGCcugaGGGCcugcccgUGGCCagGCCCa -3' miRNA: 3'- cACCGACUUG----CCUG-------ACCGGaaCGGGa -5' |
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| 19596 | 3' | -58.5 | NC_004686.1 | + | 8298 | 0.71 | 0.244465 |
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Target: 5'- cGUGGCUGGGCcuggccaccGGGCaGGCCcucagGCCCa -3' miRNA: 3'- -CACCGACUUG---------CCUGaCCGGaa---CGGGa -5' |
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| 19596 | 3' | -58.5 | NC_004686.1 | + | 53283 | 0.71 | 0.238248 |
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Target: 5'- uUGGCUGc-CGGugUGGCUUgucaucGCCCUg -3' miRNA: 3'- cACCGACuuGCCugACCGGAa-----CGGGA- -5' |
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| 19596 | 3' | -58.5 | NC_004686.1 | + | 24090 | 1.06 | 0.00064 |
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Target: 5'- gGUGGCUGAACGGACUGGCCUUGCCCUc -3' miRNA: 3'- -CACCGACUUGCCUGACCGGAACGGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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