Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19598 | 5' | -61.1 | NC_004686.1 | + | 25893 | 0.71 | 0.211726 |
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Target: 5'- -gGCCGCGGGCCGGCgcacagccaguaccaGGACgcugCGaGGa -3' miRNA: 3'- caUGGCGUCCGGCCG---------------CCUGa---GCaCCg -5' |
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| 19598 | 5' | -61.1 | NC_004686.1 | + | 908 | 0.71 | 0.209583 |
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Target: 5'- -cGCgGCAGGCCGGCaaaacguggGGugUCaUGGUc -3' miRNA: 3'- caUGgCGUCCGGCCG---------CCugAGcACCG- -5' |
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| 19598 | 5' | -61.1 | NC_004686.1 | + | 38415 | 0.71 | 0.204306 |
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Target: 5'- -cGCCGCGGGCaucauCGGCcccGGACUCGccGCa -3' miRNA: 3'- caUGGCGUCCG-----GCCG---CCUGAGCacCG- -5' |
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| 19598 | 5' | -61.1 | NC_004686.1 | + | 6993 | 0.71 | 0.204306 |
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Target: 5'- --uCCGaucGGCUGGUGGugUgGUGGCg -3' miRNA: 3'- cauGGCgu-CCGGCCGCCugAgCACCG- -5' |
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| 19598 | 5' | -61.1 | NC_004686.1 | + | 42933 | 0.72 | 0.161818 |
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Target: 5'- -cACCGCAGGCCc-CGGGCUCucGGCg -3' miRNA: 3'- caUGGCGUCCGGccGCCUGAGcaCCG- -5' |
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| 19598 | 5' | -61.1 | NC_004686.1 | + | 25658 | 1.09 | 0.000277 |
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Target: 5'- uGUACCGCAGGCCGGCGGACUCGUGGCc -3' miRNA: 3'- -CAUGGCGUCCGGCCGCCUGAGCACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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