Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19601 | 3' | -57.8 | NC_004686.1 | + | 49080 | 0.66 | 0.571467 |
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Target: 5'- gGUCAGCGAGGGagagcGCGaGCCAaacgCCAucACg -3' miRNA: 3'- -UAGUUGCUCCCa----CGC-CGGUg---GGU--UGa -5' |
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| 19601 | 3' | -57.8 | NC_004686.1 | + | 6648 | 0.66 | 0.560688 |
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Target: 5'- cGUCGACGAuGGUGaGGaCCAUCCAugaACUg -3' miRNA: 3'- -UAGUUGCUcCCACgCC-GGUGGGU---UGA- -5' |
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| 19601 | 3' | -57.8 | NC_004686.1 | + | 30039 | 0.67 | 0.477069 |
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Target: 5'- cUCAGCcGGGGUcgguguggcgGCGGCUugCCGAUc -3' miRNA: 3'- uAGUUGcUCCCA----------CGCCGGugGGUUGa -5' |
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| 19601 | 3' | -57.8 | NC_004686.1 | + | 26902 | 0.67 | 0.457111 |
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Target: 5'- -aCAAUGAcuGGGcUGCGGUCACCCu--- -3' miRNA: 3'- uaGUUGCU--CCC-ACGCCGGUGGGuuga -5' |
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| 19601 | 3' | -57.8 | NC_004686.1 | + | 39662 | 0.68 | 0.43761 |
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Target: 5'- uGUCAcgggUGAGGGUGCGGCacggcaucaGCCgCGGCg -3' miRNA: 3'- -UAGUu---GCUCCCACGCCGg--------UGG-GUUGa -5' |
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| 19601 | 3' | -57.8 | NC_004686.1 | + | 41964 | 0.68 | 0.409277 |
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Target: 5'- uUCGACGAGGaugaGCGGCCAUgggaCAGCg -3' miRNA: 3'- uAGUUGCUCCca--CGCCGGUGg---GUUGa -5' |
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| 19601 | 3' | -57.8 | NC_004686.1 | + | 24242 | 0.69 | 0.382116 |
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Target: 5'- ----uUGGGGGUG-GGCCGCCUGGCg -3' miRNA: 3'- uaguuGCUCCCACgCCGGUGGGUUGa -5' |
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| 19601 | 3' | -57.8 | NC_004686.1 | + | 26779 | 1.06 | 0.000826 |
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Target: 5'- cAUCAACGAGGGUGCGGCCACCCAACUg -3' miRNA: 3'- -UAGUUGCUCCCACGCCGGUGGGUUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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