Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19601 | 5' | -51.4 | NC_004686.1 | + | 51383 | 0.66 | 0.898855 |
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Target: 5'- cGAGUGCgauuGCGGCUGGUCCaugaACa-CUCg -3' miRNA: 3'- aCUUACG----CGUUGACUAGGg---UGcaGAG- -5' |
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| 19601 | 5' | -51.4 | NC_004686.1 | + | 54270 | 0.66 | 0.891537 |
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Target: 5'- cUGGAUGCGCAACccaaacagGAcuacagCCUugGUCg- -3' miRNA: 3'- -ACUUACGCGUUGa-------CUa-----GGGugCAGag -5' |
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| 19601 | 5' | -51.4 | NC_004686.1 | + | 52976 | 0.67 | 0.841934 |
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Target: 5'- cGAgccAUGCGCcGCaUGAUCCUggccgcccgACGUUUCa -3' miRNA: 3'- aCU---UACGCGuUG-ACUAGGG---------UGCAGAG- -5' |
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| 19601 | 5' | -51.4 | NC_004686.1 | + | 49261 | 0.69 | 0.745995 |
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Target: 5'- aGAGUGCGCGcCUGGgcgaugaacucggcgUCCC-CGUCg- -3' miRNA: 3'- aCUUACGCGUuGACU---------------AGGGuGCAGag -5' |
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| 19601 | 5' | -51.4 | NC_004686.1 | + | 10529 | 0.72 | 0.566137 |
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Target: 5'- cUGAGUGCGCAACucguuggcggugucgUGGUCcCCGCGgauaUCg -3' miRNA: 3'- -ACUUACGCGUUG---------------ACUAG-GGUGCag--AG- -5' |
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| 19601 | 5' | -51.4 | NC_004686.1 | + | 26745 | 1.09 | 0.002226 |
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Target: 5'- cUGAAUGCGCAACUGAUCCCACGUCUCc -3' miRNA: 3'- -ACUUACGCGUUGACUAGGGUGCAGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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