Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19602 | 3' | -54.6 | NC_004686.1 | + | 48236 | 0.65 | 0.790841 |
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Target: 5'- aCGACCGGcacgacaccgccUCCAG-GACACGCGg-- -3' miRNA: 3'- -GCUGGCCaa----------AGGUCaCUGUGUGCgga -5' |
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| 19602 | 3' | -54.6 | NC_004686.1 | + | 40403 | 0.66 | 0.77499 |
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Target: 5'- -uGCCGGUgagCAGUGgucACugACGCCg -3' miRNA: 3'- gcUGGCCAaagGUCAC---UGugUGCGGa -5' |
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| 19602 | 3' | -54.6 | NC_004686.1 | + | 24407 | 0.66 | 0.77499 |
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Target: 5'- -cGCCGcug-CCGGUGGCGCAUGCg- -3' miRNA: 3'- gcUGGCcaaaGGUCACUGUGUGCGga -5' |
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| 19602 | 3' | -54.6 | NC_004686.1 | + | 14507 | 0.67 | 0.733815 |
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Target: 5'- gCGAuCCGGag-CC-GUGGCGCgGCGCCa -3' miRNA: 3'- -GCU-GGCCaaaGGuCACUGUG-UGCGGa -5' |
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| 19602 | 3' | -54.6 | NC_004686.1 | + | 22148 | 0.67 | 0.701825 |
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Target: 5'- uGGCCGccgUUCgCGGUGAgCgACACGCCa -3' miRNA: 3'- gCUGGCca-AAG-GUCACU-G-UGUGCGGa -5' |
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| 19602 | 3' | -54.6 | NC_004686.1 | + | 13064 | 0.68 | 0.665942 |
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Target: 5'- -aACCGGUUUCCGG-GACuucugcuuugcgcgGCGCuGCCg -3' miRNA: 3'- gcUGGCCAAAGGUCaCUG--------------UGUG-CGGa -5' |
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| 19602 | 3' | -54.6 | NC_004686.1 | + | 52257 | 0.68 | 0.63631 |
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Target: 5'- uCGuCCGGcgaUCCAGUGAgGCA-GCCa -3' miRNA: 3'- -GCuGGCCaa-AGGUCACUgUGUgCGGa -5' |
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| 19602 | 3' | -54.6 | NC_004686.1 | + | 22223 | 0.68 | 0.63631 |
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Target: 5'- aGGCCGGUgaugacagCGGUGccgccgaggccGCGCGCGCCa -3' miRNA: 3'- gCUGGCCAaag-----GUCAC-----------UGUGUGCGGa -5' |
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| 19602 | 3' | -54.6 | NC_004686.1 | + | 27229 | 1.08 | 0.001638 |
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Target: 5'- cCGACCGGUUUCCAGUGACACACGCCUu -3' miRNA: 3'- -GCUGGCCAAAGGUCACUGUGUGCGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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