Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19610 | 5' | -59.2 | NC_004686.1 | + | 39182 | 0.66 | 0.483841 |
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Target: 5'- -aCGGGcGCaGAGgUGGGGCgcaugaccgGCCGUGUc -3' miRNA: 3'- caGCCU-CGaCUCgACCCCG---------UGGUACA- -5' |
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| 19610 | 5' | -59.2 | NC_004686.1 | + | 11798 | 0.66 | 0.444326 |
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Target: 5'- -aUGGGGCUgGGGCgaaGGGCGCCAa-- -3' miRNA: 3'- caGCCUCGA-CUCGac-CCCGUGGUaca -5' |
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| 19610 | 5' | -59.2 | NC_004686.1 | + | 49840 | 0.67 | 0.388635 |
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Target: 5'- uUCGGAGCUGGaucGGGGCAUCGc-- -3' miRNA: 3'- cAGCCUCGACUcgaCCCCGUGGUaca -5' |
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| 19610 | 5' | -59.2 | NC_004686.1 | + | 33550 | 0.7 | 0.277671 |
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Target: 5'- aUCGGAGCUGAugGCacuggUGGGGCuguuagGCCcgGUg -3' miRNA: 3'- cAGCCUCGACU--CG-----ACCCCG------UGGuaCA- -5' |
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| 19610 | 5' | -59.2 | NC_004686.1 | + | 50903 | 0.73 | 0.160394 |
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Target: 5'- cGUCGGcGUUGAaguccaGCggucGGGGCACCAUGUu -3' miRNA: 3'- -CAGCCuCGACU------CGa---CCCCGUGGUACA- -5' |
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| 19610 | 5' | -59.2 | NC_004686.1 | + | 33594 | 1.07 | 0.000445 |
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Target: 5'- cGUCGGAGCUGAGCUGGGGCACCAUGUg -3' miRNA: 3'- -CAGCCUCGACUCGACCCCGUGGUACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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