Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19617 | 3' | -56.5 | NC_004686.1 | + | 4808 | 0.66 | 0.65124 |
Target: 5'- uCUUCuUGCGCUCGgccUCGGCAUUCGc--- -3' miRNA: 3'- -GGAG-ACGCGGGC---AGCCGUGAGUaagc -5' |
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19617 | 3' | -56.5 | NC_004686.1 | + | 26009 | 0.67 | 0.640447 |
Target: 5'- cCCagcgCUGCuGCCgGUCgGGCAUUCcgUUGg -3' miRNA: 3'- -GGa---GACG-CGGgCAG-CCGUGAGuaAGC- -5' |
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19617 | 3' | -56.5 | NC_004686.1 | + | 48499 | 0.7 | 0.415023 |
Target: 5'- uCCUC-GCGCCCGUCGa-ACUCAcggCGg -3' miRNA: 3'- -GGAGaCGCGGGCAGCcgUGAGUaa-GC- -5' |
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19617 | 3' | -56.5 | NC_004686.1 | + | 39251 | 1.11 | 0.000608 |
Target: 5'- aCCUCUGCGCCCGUCGGCACUCAUUCGa -3' miRNA: 3'- -GGAGACGCGGGCAGCCGUGAGUAAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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