Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19620 | 3' | -54.6 | NC_004686.1 | + | 8668 | 0.66 | 0.729605 |
Target: 5'- -cGCcaACGCCUUCugguACUuGcGGGACUCa -3' miRNA: 3'- aaCG--UGCGGAAG----UGAuCaCCCUGAGc -5' |
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19620 | 3' | -54.6 | NC_004686.1 | + | 6531 | 0.66 | 0.729605 |
Target: 5'- aUGCACGCCgacggugaACggAGUGGGGaauCUCa -3' miRNA: 3'- aACGUGCGGaag-----UGa-UCACCCU---GAGc -5' |
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19620 | 3' | -54.6 | NC_004686.1 | + | 23987 | 0.66 | 0.718789 |
Target: 5'- cUGUAUGCCUUCACUGuGuUGaGGGCa-- -3' miRNA: 3'- aACGUGCGGAAGUGAU-C-AC-CCUGagc -5' |
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19620 | 3' | -54.6 | NC_004686.1 | + | 40245 | 1.06 | 0.001612 |
Target: 5'- uUUGCACGCCUUCACUAGUGGGACUCGu -3' miRNA: 3'- -AACGUGCGGAAGUGAUCACCCUGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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