miRNA: 3'- agACUG--ACCGCa-----GCCGu---GCGGUAGCU- -5'
miRNA: 3'- aGACu-GACCGCAGC---------CGUGCGGUAGCU- -5'
miRNA: 3'- -AGACUGa----CCGCAGCCGuGCGGUAGCU- -5'
miRNA: 3'- -AGACUGACCGcAGCCGUGCGGUAGCU- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19621 | 5' | -57.4 | NC_004686.1 | + | 41625 | 0.72 | 0.281972 |
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Target: 5'- -gUGACuuUGGCGguuuugCGGCcuuuCGCCAUCGAc -3' miRNA: 3'- agACUG--ACCGCa-----GCCGu---GCGGUAGCU- -5' |
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| 19621 | 5' | -57.4 | NC_004686.1 | + | 33468 | 0.74 | 0.202303 |
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Target: 5'- gCUGuuCUGGUGUUGugguguuucGCGCGCCGUCGAg -3' miRNA: 3'- aGACu-GACCGCAGC---------CGUGCGGUAGCU- -5' |
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| 19621 | 5' | -57.4 | NC_004686.1 | + | 34998 | 0.82 | 0.056777 |
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Target: 5'- cUCUGACgaaagGGUGUCGGU-CGCCGUCGAc -3' miRNA: 3'- -AGACUGa----CCGCAGCCGuGCGGUAGCU- -5' |
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| 19621 | 5' | -57.4 | NC_004686.1 | + | 40319 | 0.98 | 0.004018 |
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Target: 5'- uUCUGACUGGC-UCGGCACGCCAUCGAg -3' miRNA: 3'- -AGACUGACCGcAGCCGUGCGGUAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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