miRNA: 3'- -CCGUgGGAGUUGUuGuaGCUAGUC-GG- -5'
miRNA: 3'- -CCGUGG-----GA-GUUGuUGUAGCUaGUCGG- -5'
miRNA: 3'- -CCGUGgGAGUugUUGUAGCUAgUCgg -5'
Results 81 - 83 of 83 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 19623 | 3' | -50.7 | NC_004686.1 | + | 12140 | 0.68 | 0.873974 |
|
Target: 5'- gGGCAgUUUCGGCGcCggCGGUCAGgCCg -3' miRNA: 3'- -CCGUgGGAGUUGUuGuaGCUAGUC-GG- -5' |
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| 19623 | 3' | -50.7 | NC_004686.1 | + | 22393 | 0.68 | 0.865826 |
|
Target: 5'- cGGCACCgauggCUaCAACcGCAUCGAcUGGCUg -3' miRNA: 3'- -CCGUGG-----GA-GUUGuUGUAGCUaGUCGG- -5' |
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| 19623 | 3' | -50.7 | NC_004686.1 | + | 41685 | 0.66 | 0.94015 |
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Target: 5'- gGGCGCgUUCGuuGACGUCGAUgGGg- -3' miRNA: 3'- -CCGUGgGAGUugUUGUAGCUAgUCgg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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