Results 61 - 70 of 70 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 19623 | 5' | -50.9 | NC_004686.1 | + | 40311 | 0.67 | 0.90293 |
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Target: 5'- gGC-ACUUCAUCGGCGUCAGUGAccACuGCu -3' miRNA: 3'- -UGuUGGGGUAGCUGCAGUUGCU--UG-CG- -5' |
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| 19623 | 5' | -50.9 | NC_004686.1 | + | 22373 | 0.66 | 0.916153 |
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Target: 5'- gACGGCUCCAaUGGCGgcaccggCAcCGAugGCu -3' miRNA: 3'- -UGUUGGGGUaGCUGCa------GUuGCUugCG- -5' |
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| 19623 | 5' | -50.9 | NC_004686.1 | + | 18862 | 0.67 | 0.895898 |
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Target: 5'- gACAAucCCCCGUUGGugauugcgaaGUCGGCGGugGUa -3' miRNA: 3'- -UGUU--GGGGUAGCUg---------CAGUUGCUugCG- -5' |
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| 19623 | 5' | -50.9 | NC_004686.1 | + | 22478 | 0.67 | 0.895898 |
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Target: 5'- uCAGCCCCGcccCGGCGgCGAUGAcCGUc -3' miRNA: 3'- uGUUGGGGUa--GCUGCaGUUGCUuGCG- -5' |
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| 19623 | 5' | -50.9 | NC_004686.1 | + | 30430 | 0.67 | 0.888591 |
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Target: 5'- -gAACCCgGUCGAUGgUCGccACGAugcgauccaACGCa -3' miRNA: 3'- ugUUGGGgUAGCUGC-AGU--UGCU---------UGCG- -5' |
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| 19623 | 5' | -50.9 | NC_004686.1 | + | 18321 | 0.67 | 0.888591 |
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Target: 5'- cACGAUCCUucaGGUGUCGuCGAACGCg -3' miRNA: 3'- -UGUUGGGGuagCUGCAGUuGCUUGCG- -5' |
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| 19623 | 5' | -50.9 | NC_004686.1 | + | 55980 | 0.67 | 0.881013 |
|
Target: 5'- cCAACCCCucacugGUCGACcaCAGCGAcaACGg -3' miRNA: 3'- uGUUGGGG------UAGCUGcaGUUGCU--UGCg -5' |
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| 19623 | 5' | -50.9 | NC_004686.1 | + | 22325 | 0.68 | 0.868339 |
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Target: 5'- gACGGCCCCGccgguaauggcggguUCGGa-UCGGCGGGCGg -3' miRNA: 3'- -UGUUGGGGU---------------AGCUgcAGUUGCUUGCg -5' |
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| 19623 | 5' | -50.9 | NC_004686.1 | + | 23801 | 0.68 | 0.865068 |
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Target: 5'- cGCAACCCCcgcCGAUGUgAGC-GugGCu -3' miRNA: 3'- -UGUUGGGGua-GCUGCAgUUGcUugCG- -5' |
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| 19623 | 5' | -50.9 | NC_004686.1 | + | 54920 | 0.77 | 0.362747 |
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Target: 5'- aACAAguUCUCGUCGugGaCGACGGACGCa -3' miRNA: 3'- -UGUU--GGGGUAGCugCaGUUGCUUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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