miRNA: 3'- caCGGCGC--UGGUUGCAGCUUGGcCUU- -5'
miRNA: 3'- cACggCGCUGGUUGCAGCUUGGCCUu -5'
miRNA: 3'- -CACGGCGCUGGUUGCAgCUuGGCCUU- -5'
miRNA: 3'- -CACGGCGCUGGUUGCAGCUUGGCCUU- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19625 | 5' | -56.8 | NC_004686.1 | + | 43754 | 0.72 | 0.283197 |
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Target: 5'- -cGCCGCGccAUCGGCGUCGAACUcGAAg -3' miRNA: 3'- caCGGCGC--UGGUUGCAGCUUGGcCUU- -5' |
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| 19625 | 5' | -56.8 | NC_004686.1 | + | 13734 | 0.8 | 0.088692 |
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Target: 5'- cUGaaGUGAUCGACGUCGAACCGGAc -3' miRNA: 3'- cACggCGCUGGUUGCAGCUUGGCCUu -5' |
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| 19625 | 5' | -56.8 | NC_004686.1 | + | 11795 | 0.92 | 0.013001 |
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Target: 5'- cGUGCCGCGACCAACGUgGAuCCGGAAc -3' miRNA: 3'- -CACGGCGCUGGUUGCAgCUuGGCCUU- -5' |
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| 19625 | 5' | -56.8 | NC_004686.1 | + | 12479 | 1.06 | 0.001216 |
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Target: 5'- cGUGCCGCGACCAACGUCGAACCGGAAc -3' miRNA: 3'- -CACGGCGCUGGUUGCAGCUUGGCCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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