Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19627 | 3' | -51.2 | NC_004686.1 | + | 22865 | 0.67 | 0.867914 |
Target: 5'- uGGAGUGAAC-GCCGgUguUGcUGUGUGu -3' miRNA: 3'- -UCUUACUUGuCGGCgAguGC-ACACAC- -5' |
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19627 | 3' | -51.2 | NC_004686.1 | + | 7165 | 0.67 | 0.841934 |
Target: 5'- gGGAAUGAACAGCCacggcccCUCA-GUG-GUGc -3' miRNA: 3'- -UCUUACUUGUCGGc------GAGUgCACaCAC- -5' |
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19627 | 3' | -51.2 | NC_004686.1 | + | 20342 | 0.67 | 0.841934 |
Target: 5'- uGAAUGGACGGCCGCggGCGa----- -3' miRNA: 3'- uCUUACUUGUCGGCGagUGCacacac -5' |
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19627 | 3' | -51.2 | NC_004686.1 | + | 860 | 0.71 | 0.629581 |
Target: 5'- ----aGGACGGCaCGCUgGCGUGUGa- -3' miRNA: 3'- ucuuaCUUGUCG-GCGAgUGCACACac -5' |
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19627 | 3' | -51.2 | NC_004686.1 | + | 13013 | 0.74 | 0.443835 |
Target: 5'- gGGAcAUGGACuuGCaGUUCACGUGUGUGg -3' miRNA: 3'- -UCU-UACUUGu-CGgCGAGUGCACACAC- -5' |
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19627 | 3' | -51.2 | NC_004686.1 | + | 10788 | 1.08 | 0.00268 |
Target: 5'- cAGAAUGAACAGCCGCUCACGUGUGUGu -3' miRNA: 3'- -UCUUACUUGUCGGCGAGUGCACACAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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