Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19627 | 5' | -53.7 | NC_004686.1 | + | 47703 | 0.66 | 0.781992 |
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Target: 5'- cGGCGCaACGCGGcUuugaugucacGGaCACUGGCCu-- -3' miRNA: 3'- -CCGUG-UGCGCCuA----------UC-GUGACUGGuaa -5' |
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| 19627 | 5' | -53.7 | NC_004686.1 | + | 3424 | 0.66 | 0.778939 |
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Target: 5'- aGGCugAUGCGGGUaugaagcaguuggcGGCGgUG-CCGUg -3' miRNA: 3'- -CCGugUGCGCCUA--------------UCGUgACuGGUAa -5' |
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| 19627 | 5' | -53.7 | NC_004686.1 | + | 11757 | 0.67 | 0.72944 |
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Target: 5'- cGGCAUcaacguggagaACGCGGGcAGCGCcgGAuCCAUg -3' miRNA: 3'- -CCGUG-----------UGCGCCUaUCGUGa-CU-GGUAa -5' |
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| 19627 | 5' | -53.7 | NC_004686.1 | + | 17094 | 0.67 | 0.72944 |
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Target: 5'- uGGCuGCAgGUcuuucgGGAaGGCACUGACCGc- -3' miRNA: 3'- -CCG-UGUgCG------CCUaUCGUGACUGGUaa -5' |
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| 19627 | 5' | -53.7 | NC_004686.1 | + | 25570 | 0.67 | 0.696592 |
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Target: 5'- uGCGCGCGUucGGggGGCGCUGAUg--- -3' miRNA: 3'- cCGUGUGCG--CCuaUCGUGACUGguaa -5' |
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| 19627 | 5' | -53.7 | NC_004686.1 | + | 22249 | 0.68 | 0.674346 |
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Target: 5'- gGGCGCugGCGGuaagGGCggcggcggcaaGCUGuCCAa- -3' miRNA: 3'- -CCGUGugCGCCua--UCG-----------UGACuGGUaa -5' |
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| 19627 | 5' | -53.7 | NC_004686.1 | + | 48891 | 0.68 | 0.629445 |
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Target: 5'- gGGCuacgcCACGCGGAcGGCGCacggaaucUGGCCGc- -3' miRNA: 3'- -CCGu----GUGCGCCUaUCGUG--------ACUGGUaa -5' |
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| 19627 | 5' | -53.7 | NC_004686.1 | + | 21953 | 0.69 | 0.570108 |
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Target: 5'- aGGCcgacguacucaacgACGCGCGGAcucggcGGCGCUGGCgGUg -3' miRNA: 3'- -CCG--------------UGUGCGCCUa-----UCGUGACUGgUAa -5' |
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| 19627 | 5' | -53.7 | NC_004686.1 | + | 10826 | 1.07 | 0.001768 |
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Target: 5'- uGGCACACGCGGAUAGCACUGACCAUUc -3' miRNA: 3'- -CCGUGUGCGCCUAUCGUGACUGGUAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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