Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19629 | 5' | -51.1 | NC_004686.1 | + | 43653 | 0.66 | 0.876941 |
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Target: 5'- gUCGGU-CUGCuCCGGggucaccGGCugGCUGGc -3' miRNA: 3'- aAGCUAuGAUG-GGCU-------UUGugCGACCa -5' |
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| 19629 | 5' | -51.1 | NC_004686.1 | + | 37414 | 0.67 | 0.843696 |
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Target: 5'- -aCGAUGCguauCgCCGAAGCGCGCUa-- -3' miRNA: 3'- aaGCUAUGau--G-GGCUUUGUGCGAcca -5' |
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| 19629 | 5' | -51.1 | NC_004686.1 | + | 43236 | 0.68 | 0.815617 |
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Target: 5'- -cCGAgugGCUugCCGcGGACGUGCUGGUc -3' miRNA: 3'- aaGCUa--UGAugGGC-UUUGUGCGACCA- -5' |
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| 19629 | 5' | -51.1 | NC_004686.1 | + | 832 | 0.68 | 0.812701 |
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Target: 5'- aUUUGGUACgcgGCCCuGGguuugcgugaggacGGCACGCUGGc -3' miRNA: 3'- -AAGCUAUGa--UGGG-CU--------------UUGUGCGACCa -5' |
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| 19629 | 5' | -51.1 | NC_004686.1 | + | 32703 | 0.69 | 0.747719 |
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Target: 5'- aUUCGAUGCUGCCgacccagaugccguuCGA---GCGCUGGa -3' miRNA: 3'- -AAGCUAUGAUGG---------------GCUuugUGCGACCa -5' |
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| 19629 | 5' | -51.1 | NC_004686.1 | + | 7786 | 0.7 | 0.71036 |
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Target: 5'- cUCGAUGCggcGCUCGAAccGCAUgGCUGGa -3' miRNA: 3'- aAGCUAUGa--UGGGCUU--UGUG-CGACCa -5' |
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| 19629 | 5' | -51.1 | NC_004686.1 | + | 36272 | 0.77 | 0.324575 |
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Target: 5'- --aGGUACUGCCCGu-GCGgGCUGGUg -3' miRNA: 3'- aagCUAUGAUGGGCuuUGUgCGACCA- -5' |
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| 19629 | 5' | -51.1 | NC_004686.1 | + | 10146 | 1.06 | 0.003939 |
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Target: 5'- gUUCGAUACUACCCGAAACACGCUGGUa -3' miRNA: 3'- -AAGCUAUGAUGGGCUUUGUGCGACCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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