Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19632 | 5' | -56.5 | NC_004686.1 | + | 590 | 0.66 | 0.670865 |
Target: 5'- gGUUGcCCUCGCaCCCGagggugcCGUcGUCGAugGAc -3' miRNA: 3'- -UAGC-GGAGUG-GGGU-------GCA-CAGCUugCU- -5' |
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19632 | 5' | -56.5 | NC_004686.1 | + | 27817 | 0.67 | 0.585015 |
Target: 5'- uUCGCCaCACCUgGgGaUGUCGAAUGGa -3' miRNA: 3'- uAGCGGaGUGGGgUgC-ACAGCUUGCU- -5' |
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19632 | 5' | -56.5 | NC_004686.1 | + | 18578 | 0.68 | 0.552805 |
Target: 5'- cAUCGgCUCcguGCUCCAUacgGUCGAACGAa -3' miRNA: 3'- -UAGCgGAG---UGGGGUGca-CAGCUUGCU- -5' |
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19632 | 5' | -56.5 | NC_004686.1 | + | 38440 | 0.7 | 0.412379 |
Target: 5'- cUCGCCgCACCCCACGcccaggCGGACa- -3' miRNA: 3'- uAGCGGaGUGGGGUGCaca---GCUUGcu -5' |
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19632 | 5' | -56.5 | NC_004686.1 | + | 8167 | 1.06 | 0.001228 |
Target: 5'- cAUCGCCUCACCCCACGUGUCGAACGAc -3' miRNA: 3'- -UAGCGGAGUGGGGUGCACAGCUUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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