Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19634 | 3' | -59.8 | NC_004686.1 | + | 26043 | 0.66 | 0.490169 |
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Target: 5'- uGGCUCgaagaaguccuGCGCcCUgCUCUCGgUGACCGg -3' miRNA: 3'- -CCGAG-----------CGCGaGA-GGGAGCgACUGGUg -5' |
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| 19634 | 3' | -59.8 | NC_004686.1 | + | 32379 | 0.66 | 0.470406 |
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Target: 5'- -uCUCG-GCUCUCCgCUCGCcgcGGCCGa -3' miRNA: 3'- ccGAGCgCGAGAGG-GAGCGa--CUGGUg -5' |
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| 19634 | 3' | -59.8 | NC_004686.1 | + | 54473 | 0.66 | 0.460677 |
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Target: 5'- cGUgCGCGgaCUCUCCCUCGC--GCCAa -3' miRNA: 3'- cCGaGCGC--GAGAGGGAGCGacUGGUg -5' |
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| 19634 | 3' | -59.8 | NC_004686.1 | + | 5409 | 0.66 | 0.460677 |
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Target: 5'- cGGUgUCGCGCuggacaacaccuUCUCgUUCauGCUGGCCACc -3' miRNA: 3'- -CCG-AGCGCG------------AGAGgGAG--CGACUGGUG- -5' |
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| 19634 | 3' | -59.8 | NC_004686.1 | + | 37917 | 0.66 | 0.460677 |
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Target: 5'- aGGCUCGCcCgCUgCCgUGCUGACCcACc -3' miRNA: 3'- -CCGAGCGcGaGAgGGaGCGACUGG-UG- -5' |
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| 19634 | 3' | -59.8 | NC_004686.1 | + | 19054 | 0.67 | 0.441547 |
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Target: 5'- aGGCUCGUGgacUUCUUCCUCaGCcu-CCACa -3' miRNA: 3'- -CCGAGCGC---GAGAGGGAG-CGacuGGUG- -5' |
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| 19634 | 3' | -59.8 | NC_004686.1 | + | 7207 | 0.67 | 0.422874 |
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Target: 5'- uGCUuucgCGCGCUCUacaacgucgCCCUCGCguucaGCCAUg -3' miRNA: 3'- cCGA----GCGCGAGA---------GGGAGCGac---UGGUG- -5' |
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| 19634 | 3' | -59.8 | NC_004686.1 | + | 15479 | 0.67 | 0.413717 |
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Target: 5'- cGGCuuUCGCGUcgUCCCUCGCgguaAUCACc -3' miRNA: 3'- -CCG--AGCGCGagAGGGAGCGac--UGGUG- -5' |
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| 19634 | 3' | -59.8 | NC_004686.1 | + | 34371 | 0.67 | 0.395776 |
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Target: 5'- cGCa-GCGCUCgCUCUCGaaGGCCGCa -3' miRNA: 3'- cCGagCGCGAGaGGGAGCgaCUGGUG- -5' |
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| 19634 | 3' | -59.8 | NC_004686.1 | + | 7117 | 1.1 | 0.000323 |
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Target: 5'- uGGCUCGCGCUCUCCCUCGCUGACCACc -3' miRNA: 3'- -CCGAGCGCGAGAGGGAGCGACUGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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