Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
19637 | 5' | -52.8 | NC_004686.1 | + | 17346 | 0.66 | 0.800491 |
Target: 5'- gGCuGCACGGg-GCAUUCCCaGCAGa- -3' miRNA: 3'- gUG-UGUGCCaaCGUGAGGGaCGUUac -5' |
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19637 | 5' | -52.8 | NC_004686.1 | + | 47104 | 0.67 | 0.790516 |
Target: 5'- -cCGC-CGcGUUGCcaguucGCUCCCUGCAAc- -3' miRNA: 3'- guGUGuGC-CAACG------UGAGGGACGUUac -5' |
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19637 | 5' | -52.8 | NC_004686.1 | + | 45528 | 0.69 | 0.638147 |
Target: 5'- gUACAUGCGGUUGCGCagCCUGa---- -3' miRNA: 3'- -GUGUGUGCCAACGUGagGGACguuac -5' |
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19637 | 5' | -52.8 | NC_004686.1 | + | 6150 | 1.09 | 0.001598 |
Target: 5'- cCACACACGGUUGCACUCCCUGCAAUGa -3' miRNA: 3'- -GUGUGUGCCAACGUGAGGGACGUUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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