Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19638 | 3' | -57.2 | NC_004686.1 | + | 49032 | 0.66 | 0.608091 |
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Target: 5'- uGAGGGGCCguGGCuGUUcAUucccggcggcagcGAGGACGc -3' miRNA: 3'- -CUCCUCGGguCCG-CAGaUA-------------CUCCUGU- -5' |
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| 19638 | 3' | -57.2 | NC_004686.1 | + | 7 | 0.68 | 0.491757 |
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Target: 5'- uGAGGGGCUCugGGGgGUCgggGUcGGGGACc -3' miRNA: 3'- -CUCCUCGGG--UCCgCAGa--UA-CUCCUGu -5' |
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| 19638 | 3' | -57.2 | NC_004686.1 | + | 47648 | 0.69 | 0.393952 |
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Target: 5'- -cGGAGCCgCGGGUGUUUGUGcggccgaAGGugAa -3' miRNA: 3'- cuCCUCGG-GUCCGCAGAUAC-------UCCugU- -5' |
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| 19638 | 3' | -57.2 | NC_004686.1 | + | 2407 | 0.7 | 0.342958 |
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Target: 5'- -uGGAGC--GGGUGUCgGUGAGGACGa -3' miRNA: 3'- cuCCUCGggUCCGCAGaUACUCCUGU- -5' |
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| 19638 | 3' | -57.2 | NC_004686.1 | + | 19054 | 0.7 | 0.342136 |
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Target: 5'- cGGGugcuGGCCggCAGGCGUCUuuggcgcaguucgGUGAGGGCGg -3' miRNA: 3'- cUCC----UCGG--GUCCGCAGA-------------UACUCCUGU- -5' |
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| 19638 | 3' | -57.2 | NC_004686.1 | + | 5662 | 1.08 | 0.000711 |
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Target: 5'- gGAGGAGCCCAGGCGUCUAUGAGGACAu -3' miRNA: 3'- -CUCCUCGGGUCCGCAGAUACUCCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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