Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19639 | 3' | -57.7 | NC_004686.1 | + | 53738 | 0.66 | 0.564562 |
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Target: 5'- aACCUCggugCCACCAagacuGUCCAGGUc -3' miRNA: 3'- cUGGAGaug-GGUGGUgu---CAGGUCCGa -5' |
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| 19639 | 3' | -57.7 | NC_004686.1 | + | 25158 | 0.67 | 0.501246 |
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Target: 5'- cGACUUCgACCCcaucaucaaaACCGCcGUCgAGGCUg -3' miRNA: 3'- -CUGGAGaUGGG----------UGGUGuCAGgUCCGA- -5' |
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| 19639 | 3' | -57.7 | NC_004686.1 | + | 39205 | 0.68 | 0.412813 |
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Target: 5'- cGCCg--ACCCGCCgGCGG-CCAGGCc -3' miRNA: 3'- cUGGagaUGGGUGG-UGUCaGGUCCGa -5' |
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| 19639 | 3' | -57.7 | NC_004686.1 | + | 43323 | 0.69 | 0.368028 |
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Target: 5'- cACC-CUGCCgACCGCGGUCCucGCc -3' miRNA: 3'- cUGGaGAUGGgUGGUGUCAGGucCGa -5' |
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| 19639 | 3' | -57.7 | NC_004686.1 | + | 40652 | 0.7 | 0.334678 |
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Target: 5'- -uCCUCUguggGCCgagCACCGCAGUCCcacAGGCg -3' miRNA: 3'- cuGGAGA----UGG---GUGGUGUCAGG---UCCGa -5' |
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| 19639 | 3' | -57.7 | NC_004686.1 | + | 42917 | 0.7 | 0.318851 |
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Target: 5'- aGACgCUaCUGCacaCCACCGCAGgccCCGGGCUc -3' miRNA: 3'- -CUG-GA-GAUG---GGUGGUGUCa--GGUCCGA- -5' |
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| 19639 | 3' | -57.7 | NC_004686.1 | + | 5070 | 1.07 | 0.000665 |
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Target: 5'- uGACCUCUACCCACCACAGUCCAGGCUg -3' miRNA: 3'- -CUGGAGAUGGGUGGUGUCAGGUCCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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