Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19640 | 3' | -55.8 | NC_004686.1 | + | 47653 | 0.66 | 0.689147 |
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Target: 5'- cCGCGGGUGUUU---GugCGGCCGa- -3' miRNA: 3'- aGCGCUCACAAGuacCugGUCGGCgu -5' |
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| 19640 | 3' | -55.8 | NC_004686.1 | + | 29849 | 0.66 | 0.667329 |
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Target: 5'- -gGCGGG-Gg--GUGGGCCAGgCGCAu -3' miRNA: 3'- agCGCUCaCaagUACCUGGUCgGCGU- -5' |
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| 19640 | 3' | -55.8 | NC_004686.1 | + | 41936 | 0.66 | 0.656365 |
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Target: 5'- -aGCGGguuGUGUUCccGGAUCAGCUGgAg -3' miRNA: 3'- agCGCU---CACAAGuaCCUGGUCGGCgU- -5' |
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| 19640 | 3' | -55.8 | NC_004686.1 | + | 5554 | 0.67 | 0.645379 |
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Target: 5'- -gGCGAucaGggCGUGGACCugAGCCGCc -3' miRNA: 3'- agCGCUca-CaaGUACCUGG--UCGGCGu -5' |
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| 19640 | 3' | -55.8 | NC_004686.1 | + | 25720 | 0.67 | 0.612389 |
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Target: 5'- aUCGCGGGUGaggcaUUCAcGG-CCGGUgCGCAg -3' miRNA: 3'- -AGCGCUCAC-----AAGUaCCuGGUCG-GCGU- -5' |
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| 19640 | 3' | -55.8 | NC_004686.1 | + | 9154 | 0.68 | 0.568681 |
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Target: 5'- cUCGCGGGUGagcuucUCAaccUGGacuGCCAGCCGa- -3' miRNA: 3'- -AGCGCUCACa-----AGU---ACC---UGGUCGGCgu -5' |
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| 19640 | 3' | -55.8 | NC_004686.1 | + | 25386 | 0.7 | 0.415479 |
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Target: 5'- cCGUGGGUGUggacguacaagCAuUGGACCGGUCGUg -3' miRNA: 3'- aGCGCUCACAa----------GU-ACCUGGUCGGCGu -5' |
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| 19640 | 3' | -55.8 | NC_004686.1 | + | 4803 | 1.09 | 0.000816 |
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Target: 5'- aUCGCGAGUGUUCAUGGACCAGCCGCAa -3' miRNA: 3'- -AGCGCUCACAAGUACCUGGUCGGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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