Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19642 | 5' | -51.9 | NC_004686.1 | + | 54598 | 0.7 | 0.663154 |
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Target: 5'- uGGUCCcgCGGaacaACCGCCAGCGucucguUCGUCu -3' miRNA: 3'- uCUAGGa-GCUg---UGGUGGUUGU------AGCAG- -5' |
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| 19642 | 5' | -51.9 | NC_004686.1 | + | 3493 | 0.72 | 0.584576 |
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Target: 5'- uGAUCggUUUGACACC-CCAGCAggcgCGUCg -3' miRNA: 3'- uCUAG--GAGCUGUGGuGGUUGUa---GCAG- -5' |
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| 19642 | 5' | -51.9 | NC_004686.1 | + | 51967 | 0.72 | 0.573439 |
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Target: 5'- ---gCCUCGACGCCGUCGACAUCaUCc -3' miRNA: 3'- ucuaGGAGCUGUGGUGGUUGUAGcAG- -5' |
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| 19642 | 5' | -51.9 | NC_004686.1 | + | 43853 | 0.72 | 0.540363 |
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Target: 5'- cGGUCgUCGugGCCGCCGGCAUa--- -3' miRNA: 3'- uCUAGgAGCugUGGUGGUUGUAgcag -5' |
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| 19642 | 5' | -51.9 | NC_004686.1 | + | 34208 | 0.74 | 0.426093 |
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Target: 5'- cGGAauacgCCUUGGCACCGCCAGCGcCGcCg -3' miRNA: 3'- -UCUa----GGAGCUGUGGUGGUUGUaGCaG- -5' |
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| 19642 | 5' | -51.9 | NC_004686.1 | + | 55214 | 0.81 | 0.173388 |
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Target: 5'- -aGUCCaUCGGCGCCGUCAACAUCGUCu -3' miRNA: 3'- ucUAGG-AGCUGUGGUGGUUGUAGCAG- -5' |
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| 19642 | 5' | -51.9 | NC_004686.1 | + | 5296 | 1.11 | 0.001715 |
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Target: 5'- aAGAUCCUCGACACCACCAACAUCGUCg -3' miRNA: 3'- -UCUAGGAGCUGUGGUGGUUGUAGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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