Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19643 | 3' | -53.2 | NC_004686.1 | + | 26807 | 0.67 | 0.754391 |
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Target: 5'- uAUgUUGGguuGGGAGAggGUGACCGCa -3' miRNA: 3'- -UAgGACUaucCCCUCUagUACUGGCG- -5' |
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| 19643 | 3' | -53.2 | NC_004686.1 | + | 17580 | 0.68 | 0.711365 |
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Target: 5'- -aCCUGGUAGGGGgucAGGUCAcuguUGAUguagaucuguugCGCg -3' miRNA: 3'- uaGGACUAUCCCC---UCUAGU----ACUG------------GCG- -5' |
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| 19643 | 3' | -53.2 | NC_004686.1 | + | 39778 | 0.68 | 0.667038 |
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Target: 5'- cGUCCgUGAUGGGcGGGGcuAUCGgcuuguggGACCGUg -3' miRNA: 3'- -UAGG-ACUAUCC-CCUC--UAGUa-------CUGGCG- -5' |
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| 19643 | 3' | -53.2 | NC_004686.1 | + | 7658 | 0.69 | 0.633393 |
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Target: 5'- -gCCUGAaAGGGGcgcgAGccAUCAUGGCCGa -3' miRNA: 3'- uaGGACUaUCCCC----UC--UAGUACUGGCg -5' |
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| 19643 | 3' | -53.2 | NC_004686.1 | + | 50344 | 0.72 | 0.449611 |
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Target: 5'- uGUCCgu-UGGGGGcGAUCAgagUGACCGUc -3' miRNA: 3'- -UAGGacuAUCCCCuCUAGU---ACUGGCG- -5' |
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| 19643 | 3' | -53.2 | NC_004686.1 | + | 5810 | 1.11 | 0.001141 |
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Target: 5'- aAUCCUGAUAGGGGAGAUCAUGACCGCg -3' miRNA: 3'- -UAGGACUAUCCCCUCUAGUACUGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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