Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19644 | 3' | -58.7 | NC_004686.1 | + | 2687 | 0.66 | 0.543072 |
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Target: 5'- -gUGGCGUuCCuGC-UUGCcGCGGUGa -3' miRNA: 3'- cgACCGCAuGGuCGcAGCGaCGCCAC- -5' |
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| 19644 | 3' | -58.7 | NC_004686.1 | + | 31205 | 0.66 | 0.532635 |
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Target: 5'- --cGuGCG-GCCGuCGUCGCUGCGGg- -3' miRNA: 3'- cgaC-CGCaUGGUcGCAGCGACGCCac -5' |
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| 19644 | 3' | -58.7 | NC_004686.1 | + | 15580 | 0.66 | 0.522272 |
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Target: 5'- cCUGGUcgaacGUGgCGGCGUCGCaacCGGUGu -3' miRNA: 3'- cGACCG-----CAUgGUCGCAGCGac-GCCAC- -5' |
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| 19644 | 3' | -58.7 | NC_004686.1 | + | 37709 | 0.66 | 0.502803 |
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Target: 5'- cUUGGCGgcacgauaggugaugGCCAGCGUCGCguucUGCGu-- -3' miRNA: 3'- cGACCGCa--------------UGGUCGCAGCG----ACGCcac -5' |
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| 19644 | 3' | -58.7 | NC_004686.1 | + | 190 | 0.68 | 0.414585 |
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Target: 5'- gGCgggugGGCGacggUGCCGuUGUCGCUGUGGUc -3' miRNA: 3'- -CGa----CCGC----AUGGUcGCAGCGACGCCAc -5' |
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| 19644 | 3' | -58.7 | NC_004686.1 | + | 43090 | 0.68 | 0.405477 |
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Target: 5'- cUUGGCGU-CCAG-GUCGUaGCGGUc -3' miRNA: 3'- cGACCGCAuGGUCgCAGCGaCGCCAc -5' |
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| 19644 | 3' | -58.7 | NC_004686.1 | + | 49073 | 0.69 | 0.370334 |
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Target: 5'- cGCUGGUacGCCAGCGUCcUUGUGGc- -3' miRNA: 3'- -CGACCGcaUGGUCGCAGcGACGCCac -5' |
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| 19644 | 3' | -58.7 | NC_004686.1 | + | 3145 | 0.69 | 0.370334 |
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Target: 5'- gGUUGGCGaaacuguucGCCcgugaGGCGUCGCUuccggauacgGCGGUGg -3' miRNA: 3'- -CGACCGCa--------UGG-----UCGCAGCGA----------CGCCAC- -5' |
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| 19644 | 3' | -58.7 | NC_004686.1 | + | 35306 | 0.7 | 0.337331 |
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Target: 5'- aGCUGGCugaggcUGCCGGUGaCuaUGCGGUGu -3' miRNA: 3'- -CGACCGc-----AUGGUCGCaGcgACGCCAC- -5' |
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| 19644 | 3' | -58.7 | NC_004686.1 | + | 7133 | 1.1 | 0.000425 |
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Target: 5'- cGCUGGCGUACCAGCGUCGCUGCGGUGu -3' miRNA: 3'- -CGACCGCAUGGUCGCAGCGACGCCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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