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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19646 | 5' | -56.9 | NC_004686.1 | + | 36819 | 0.66 | 0.643973 |
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Target: 5'- aCGUGGAucgcccaUCGGuUGGCAcGUUUCGCGuGGGa -3' miRNA: 3'- -GUACCU-------GGCC-ACCGU-CAAGGUGC-CUC- -5' |
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| 19646 | 5' | -56.9 | NC_004686.1 | + | 26503 | 0.67 | 0.547944 |
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Target: 5'- aGUGGGCCGGUgcugcgcaaGGCAaGUgggaUCCGgGGAa -3' miRNA: 3'- gUACCUGGCCA---------CCGU-CA----AGGUgCCUc -5' |
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| 19646 | 5' | -56.9 | NC_004686.1 | + | 11329 | 0.68 | 0.537374 |
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Target: 5'- gGUGGAguugaagcauuUCGGUGGUucGGUUCCACcGGuGa -3' miRNA: 3'- gUACCU-----------GGCCACCG--UCAAGGUG-CCuC- -5' |
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| 19646 | 5' | -56.9 | NC_004686.1 | + | 8825 | 0.7 | 0.408439 |
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Target: 5'- --gGGAUCGGgugucuGCGGUUCCGCuGGGGg -3' miRNA: 3'- guaCCUGGCCac----CGUCAAGGUG-CCUC- -5' |
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| 19646 | 5' | -56.9 | NC_004686.1 | + | 7570 | 1.09 | 0.000722 |
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Target: 5'- aCAUGGACCGGUGGCAGUUCCACGGAGa -3' miRNA: 3'- -GUACCUGGCCACCGUCAAGGUGCCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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