Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19647 | 5' | -57.3 | NC_004686.1 | + | 2591 | 0.66 | 0.572577 |
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Target: 5'- gGGCGuacugcuguaccGUCACCUGCUaUGAGUgGGGa -3' miRNA: 3'- aCCGC------------CAGUGGACGAcGCUCAgCUUa -5' |
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| 19647 | 5' | -57.3 | NC_004686.1 | + | 6327 | 0.66 | 0.561754 |
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Target: 5'- cGGCcuucGUCGCCg---GCGAGUCGggUu -3' miRNA: 3'- aCCGc---CAGUGGacgaCGCUCAGCuuA- -5' |
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| 19647 | 5' | -57.3 | NC_004686.1 | + | 413 | 0.67 | 0.508629 |
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Target: 5'- gGGCGGUCaACCg---GCGGGUcCGAGa -3' miRNA: 3'- aCCGCCAG-UGGacgaCGCUCA-GCUUa -5' |
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| 19647 | 5' | -57.3 | NC_004686.1 | + | 25085 | 0.67 | 0.508629 |
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Target: 5'- -aGUGGUCAaCUGCcGCGAgGUCGAAc -3' miRNA: 3'- acCGCCAGUgGACGaCGCU-CAGCUUa -5' |
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| 19647 | 5' | -57.3 | NC_004686.1 | + | 24521 | 0.69 | 0.419057 |
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Target: 5'- cGGCGG-CGacggUGCUGCG-GUCGAAUg -3' miRNA: 3'- aCCGCCaGUgg--ACGACGCuCAGCUUA- -5' |
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| 19647 | 5' | -57.3 | NC_004686.1 | + | 7921 | 1.05 | 0.001057 |
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Target: 5'- gUGGCGGUCACCUGCUGCGAGUCGAAUc -3' miRNA: 3'- -ACCGCCAGUGGACGACGCUCAGCUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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