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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19649 | 3' | -52.3 | NC_004686.1 | + | 14888 | 0.66 | 0.863177 |
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Target: 5'- uGGGUGGUCUcgcccaGAUCGGguccuUGGGCGGcacggUCg -3' miRNA: 3'- -UCCACCAGAa-----CUGGCU-----ACUCGUCa----AG- -5' |
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| 19649 | 3' | -52.3 | NC_004686.1 | + | 31735 | 0.66 | 0.863177 |
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Target: 5'- cGGUGGUCUcGACaUGGUG-GCGGc-- -3' miRNA: 3'- uCCACCAGAaCUG-GCUACuCGUCaag -5' |
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| 19649 | 3' | -52.3 | NC_004686.1 | + | 19045 | 0.66 | 0.85468 |
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Target: 5'- uGGGcgGGUCgggugcUGGCCGGcaggcgucuuUGGcGCAGUUCg -3' miRNA: 3'- -UCCa-CCAGa-----ACUGGCU----------ACU-CGUCAAG- -5' |
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| 19649 | 3' | -52.3 | NC_004686.1 | + | 19800 | 0.66 | 0.845937 |
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Target: 5'- uGGaGGUCgccACCGAUGAGgUGGUUCc -3' miRNA: 3'- uCCaCCAGaacUGGCUACUC-GUCAAG- -5' |
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| 19649 | 3' | -52.3 | NC_004686.1 | + | 41706 | 0.66 | 0.845937 |
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Target: 5'- uGG-GGUUgUGAUCGAUGGGCuGaUCg -3' miRNA: 3'- uCCaCCAGaACUGGCUACUCGuCaAG- -5' |
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| 19649 | 3' | -52.3 | NC_004686.1 | + | 22604 | 0.67 | 0.818324 |
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Target: 5'- cGGUGGUCacccccACCGccGGGCAGaUCg -3' miRNA: 3'- uCCACCAGaac---UGGCuaCUCGUCaAG- -5' |
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| 19649 | 3' | -52.3 | NC_004686.1 | + | 8746 | 1.1 | 0.001632 |
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Target: 5'- aAGGUGGUCUUGACCGAUGAGCAGUUCu -3' miRNA: 3'- -UCCACCAGAACUGGCUACUCGUCAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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