Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19649 | 5' | -55.5 | NC_004686.1 | + | 8030 | 0.66 | 0.705504 |
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Target: 5'- -cGAugagGCGCCgcugCCGCGGAaGUCGUu -3' miRNA: 3'- cuCUuga-CGUGGa---GGCGCCUcUAGCG- -5' |
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| 19649 | 5' | -55.5 | NC_004686.1 | + | 51093 | 0.67 | 0.673005 |
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Target: 5'- -uGAcCUGCACCU-UGCGgGGGAUgGCg -3' miRNA: 3'- cuCUuGACGUGGAgGCGC-CUCUAgCG- -5' |
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| 19649 | 5' | -55.5 | NC_004686.1 | + | 31663 | 0.67 | 0.629182 |
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Target: 5'- aGAGGACUGUugCguuccagCCgGCGGGGc-CGCg -3' miRNA: 3'- -CUCUUGACGugGa------GG-CGCCUCuaGCG- -5' |
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| 19649 | 5' | -55.5 | NC_004686.1 | + | 55708 | 0.68 | 0.578867 |
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Target: 5'- -cGAAuCUGCGCCUCCgccagcucaucaccaGCGGccAUCGCc -3' miRNA: 3'- cuCUU-GACGUGGAGG---------------CGCCucUAGCG- -5' |
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| 19649 | 5' | -55.5 | NC_004686.1 | + | 45030 | 0.68 | 0.574523 |
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Target: 5'- cGGGGAUcGCACCggCGCGGA--UCGCc -3' miRNA: 3'- -CUCUUGaCGUGGagGCGCCUcuAGCG- -5' |
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| 19649 | 5' | -55.5 | NC_004686.1 | + | 10577 | 0.68 | 0.563698 |
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Target: 5'- -uGGGCUGCgaagacgaaaGCCgCCGCGcAGGUCGCc -3' miRNA: 3'- cuCUUGACG----------UGGaGGCGCcUCUAGCG- -5' |
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| 19649 | 5' | -55.5 | NC_004686.1 | + | 43049 | 0.69 | 0.531584 |
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Target: 5'- cGGGuucCUGaCAUUUCCGUGGAGA-CGCu -3' miRNA: 3'- -CUCuu-GAC-GUGGAGGCGCCUCUaGCG- -5' |
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| 19649 | 5' | -55.5 | NC_004686.1 | + | 47407 | 0.72 | 0.384143 |
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Target: 5'- -uGGcCUGCGCgauCUCCGCGGAGGU-GCa -3' miRNA: 3'- cuCUuGACGUG---GAGGCGCCUCUAgCG- -5' |
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| 19649 | 5' | -55.5 | NC_004686.1 | + | 1991 | 0.72 | 0.375304 |
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Target: 5'- -uGAACUGCugU---GCGGGGGUCGCg -3' miRNA: 3'- cuCUUGACGugGaggCGCCUCUAGCG- -5' |
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| 19649 | 5' | -55.5 | NC_004686.1 | + | 8781 | 1.12 | 0.000606 |
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Target: 5'- gGAGAACUGCACCUCCGCGGAGAUCGCg -3' miRNA: 3'- -CUCUUGACGUGGAGGCGCCUCUAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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