miRNA: 3'- cUAGGuaCAUaCC-CCgACCCCGUCUc -5'
miRNA: 3'- cUAGG--UACAUACC-CCGAC-C-CCguCUc -5'
miRNA: 3'- cUAGGU--ACA---UACCCC-GACCCCGucuc -5'
miRNA: 3'- -CUAGGUACAUACCCCGACCCCG--UCUC- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19655 | 5' | -56.7 | NC_004686.1 | + | 32499 | 0.66 | 0.602647 |
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Target: 5'- aGUCCcgGUA-GGuGG-UGGGGCGGAu -3' miRNA: 3'- cUAGGuaCAUaCC-CCgACCCCGUCUc -5' |
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| 19655 | 5' | -56.7 | NC_004686.1 | + | 25868 | 0.66 | 0.602647 |
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Target: 5'- uGUCCugAUGUGUGGuGGCUGuGaGGguGAu -3' miRNA: 3'- cUAGG--UACAUACC-CCGAC-C-CCguCUc -5' |
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| 19655 | 5' | -56.7 | NC_004686.1 | + | 13843 | 0.67 | 0.53738 |
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Target: 5'- cAUCCGauUGUgggAUGGGGaCUGGGGCu--- -3' miRNA: 3'- cUAGGU--ACA---UACCCC-GACCCCGucuc -5' |
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| 19655 | 5' | -56.7 | NC_004686.1 | + | 11788 | 0.97 | 0.00468 |
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Target: 5'- gGAUCCAUGUAUGGGGCUGGGGCgaAGGGc -3' miRNA: 3'- -CUAGGUACAUACCCCGACCCCG--UCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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