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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19657 | 3' | -50.7 | NC_004686.1 | + | 33569 | 0.66 | 0.929712 |
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Target: 5'- gUGGGGcUGUUAGG--CCCGGUGUUc -3' miRNA: 3'- uGCCUUuACAGUCCuuGGGCCAUAGa -5' |
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| 19657 | 3' | -50.7 | NC_004686.1 | + | 18603 | 0.66 | 0.927972 |
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Target: 5'- uACGGGugcgcGUGUgCGGGugcaucacucaccgAACCUGGUGUCg -3' miRNA: 3'- -UGCCUu----UACA-GUCC--------------UUGGGCCAUAGa -5' |
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| 19657 | 3' | -50.7 | NC_004686.1 | + | 19888 | 0.66 | 0.91761 |
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Target: 5'- cAUGGccGAUGgugCGGG-GCCUGGUGUCg -3' miRNA: 3'- -UGCCu-UUACa--GUCCuUGGGCCAUAGa -5' |
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| 19657 | 3' | -50.7 | NC_004686.1 | + | 42618 | 0.67 | 0.882285 |
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Target: 5'- uGCGGGAGUuggaGUCGGuucaGGACCgGGUGUUg -3' miRNA: 3'- -UGCCUUUA----CAGUC----CUUGGgCCAUAGa -5' |
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| 19657 | 3' | -50.7 | NC_004686.1 | + | 22436 | 0.69 | 0.802263 |
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Target: 5'- gACGGggGUGUguGauGCCCGGauggAUCg -3' miRNA: 3'- -UGCCuuUACAguCcuUGGGCCa---UAGa -5' |
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| 19657 | 3' | -50.7 | NC_004686.1 | + | 3381 | 0.71 | 0.707089 |
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Target: 5'- gAUGcGAAGUGgCGGGAuCCgCGGUAUCUg -3' miRNA: 3'- -UGC-CUUUACaGUCCUuGG-GCCAUAGA- -5' |
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| 19657 | 3' | -50.7 | NC_004686.1 | + | 13152 | 1.09 | 0.003282 |
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Target: 5'- cACGGAAAUGUCAGGAACCCGGUAUCUg -3' miRNA: 3'- -UGCCUUUACAGUCCUUGGGCCAUAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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