Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19662 | 3' | -57.2 | NC_004686.1 | + | 6688 | 0.66 | 0.636661 |
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Target: 5'- gCUUCCUCc-CCUGUGGUugUGAUCCAa- -3' miRNA: 3'- -GGAGGGGuuGGACGCCA--ACUGGGUgu -5' |
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| 19662 | 3' | -57.2 | NC_004686.1 | + | 26197 | 0.67 | 0.582725 |
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Target: 5'- gCCgCCCCGACCUcGCa---GGCCCACu -3' miRNA: 3'- -GGaGGGGUUGGA-CGccaaCUGGGUGu -5' |
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| 19662 | 3' | -57.2 | NC_004686.1 | + | 6232 | 0.67 | 0.540234 |
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Target: 5'- aCUCCCCGuACggcGcCGGUcGGCCCGCAu -3' miRNA: 3'- gGAGGGGU-UGga-C-GCCAaCUGGGUGU- -5' |
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| 19662 | 3' | -57.2 | NC_004686.1 | + | 15690 | 0.68 | 0.529766 |
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Target: 5'- cCCUCCgCGACCUaGUGcGUUGACgaCGCu -3' miRNA: 3'- -GGAGGgGUUGGA-CGC-CAACUGg-GUGu -5' |
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| 19662 | 3' | -57.2 | NC_004686.1 | + | 7097 | 0.68 | 0.509062 |
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Target: 5'- --cCCCCGACCgugaugGCGuUUGGCUCGCGc -3' miRNA: 3'- ggaGGGGUUGGa-----CGCcAACUGGGUGU- -5' |
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| 19662 | 3' | -57.2 | NC_004686.1 | + | 55782 | 0.68 | 0.509062 |
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Target: 5'- uCCUCUCgGACCcGcCGGUUGACCg--- -3' miRNA: 3'- -GGAGGGgUUGGaC-GCCAACUGGgugu -5' |
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| 19662 | 3' | -57.2 | NC_004686.1 | + | 26368 | 0.68 | 0.498837 |
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Target: 5'- cCCgCCCCGACCUGCccaaGGgcuacGACCC-CGc -3' miRNA: 3'- -GGaGGGGUUGGACG----CCaa---CUGGGuGU- -5' |
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| 19662 | 3' | -57.2 | NC_004686.1 | + | 54844 | 0.69 | 0.439599 |
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Target: 5'- gUUCCgUGACC-GCGGUaagGACCCGCGc -3' miRNA: 3'- gGAGGgGUUGGaCGCCAa--CUGGGUGU- -5' |
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| 19662 | 3' | -57.2 | NC_004686.1 | + | 32467 | 0.73 | 0.242458 |
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Target: 5'- aCUgCCCAGCgUGCGGUgaucccgGACCCGgAa -3' miRNA: 3'- gGAgGGGUUGgACGCCAa------CUGGGUgU- -5' |
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| 19662 | 3' | -57.2 | NC_004686.1 | + | 14711 | 1.1 | 0.000595 |
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Target: 5'- uCCUCCCCAACCUGCGGUUGACCCACAa -3' miRNA: 3'- -GGAGGGGUUGGACGCCAACUGGGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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