miRNA: 3'- -CUCCUGUCGGacaaGCUAGg----CAACAG- -5'
miRNA: 3'- cuCCUGUCGGACa--AGCU----AGGCAACag -5'
miRNA: 3'- cUCCUGUCGGaCaagCUAGGCaaCAG- -5'
miRNA: 3'- cuCCUGUCGGACaaGCUAGGCaa--CAG- -5'
miRNA: 3'- -CUCCUGUCGGACAAGCUAGGCAACAG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19665 | 3' | -52.8 | NC_004686.1 | + | 45126 | 0.66 | 0.849842 |
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Target: 5'- cGGGGGCAGCCa---UGAUCaggguGUUGUCu -3' miRNA: 3'- -CUCCUGUCGGacaaGCUAGg----CAACAG- -5' |
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| 19665 | 3' | -52.8 | NC_004686.1 | + | 30298 | 0.66 | 0.841025 |
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Target: 5'- -uGGGCAGUUUGguuUCGAuccaUCCGUUGa- -3' miRNA: 3'- cuCCUGUCGGACa--AGCU----AGGCAACag -5' |
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| 19665 | 3' | -52.8 | NC_004686.1 | + | 7276 | 0.67 | 0.752624 |
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Target: 5'- aGGGACAGCCgGg--GAUCCGg-GUCu -3' miRNA: 3'- cUCCUGUCGGaCaagCUAGGCaaCAG- -5' |
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| 19665 | 3' | -52.8 | NC_004686.1 | + | 5696 | 0.69 | 0.687408 |
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Target: 5'- -cGGACGGUCUGggUGAUCUGaagcGUCa -3' miRNA: 3'- cuCCUGUCGGACaaGCUAGGCaa--CAG- -5' |
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| 19665 | 3' | -52.8 | NC_004686.1 | + | 16599 | 1.1 | 0.001399 |
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Target: 5'- uGAGGACAGCCUGUUCGAUCCGUUGUCg -3' miRNA: 3'- -CUCCUGUCGGACAAGCUAGGCAACAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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