Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19665 | 5' | -52.1 | NC_004686.1 | + | 19041 | 0.66 | 0.887419 |
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Target: 5'- gGUGUgGgCGggUCG-GGUGCUGgCCGg -3' miRNA: 3'- -CAUAgUgGCuuGGCuCUACGACaGGC- -5' |
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| 19665 | 5' | -52.1 | NC_004686.1 | + | 5888 | 0.66 | 0.887419 |
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Target: 5'- -gGUCGCCGAGuCCaagaaGGAUGCgcGUCUGg -3' miRNA: 3'- caUAGUGGCUU-GGc----UCUACGa-CAGGC- -5' |
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| 19665 | 5' | -52.1 | NC_004686.1 | + | 11541 | 0.66 | 0.871903 |
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Target: 5'- -cAUCuCCGAugUGAgGAUGauCUGUCCGu -3' miRNA: 3'- caUAGuGGCUugGCU-CUAC--GACAGGC- -5' |
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| 19665 | 5' | -52.1 | NC_004686.1 | + | 36322 | 0.66 | 0.855357 |
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Target: 5'- -gGUCGCCGAgGuuGAGcgGCUgcGUCCc -3' miRNA: 3'- caUAGUGGCU-UggCUCuaCGA--CAGGc -5' |
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| 19665 | 5' | -52.1 | NC_004686.1 | + | 25066 | 0.67 | 0.837842 |
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Target: 5'- -aAUgACUGAG-CGGGGUGUUGUCCc -3' miRNA: 3'- caUAgUGGCUUgGCUCUACGACAGGc -5' |
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| 19665 | 5' | -52.1 | NC_004686.1 | + | 30348 | 0.67 | 0.837842 |
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Target: 5'- aUGUCACCGAcgucggcgaaACCGuuGAU-CUGUCCc -3' miRNA: 3'- cAUAGUGGCU----------UGGCu-CUAcGACAGGc -5' |
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| 19665 | 5' | -52.1 | NC_004686.1 | + | 52197 | 0.67 | 0.809914 |
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Target: 5'- ---gCACCGGGCCG-GGUGCg--CCGc -3' miRNA: 3'- cauaGUGGCUUGGCuCUACGacaGGC- -5' |
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| 19665 | 5' | -52.1 | NC_004686.1 | + | 18630 | 0.68 | 0.800204 |
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Target: 5'- --cUCACCGAACCuGGugucgcUGCUGccUCCGg -3' miRNA: 3'- cauAGUGGCUUGGcUCu-----ACGAC--AGGC- -5' |
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| 19665 | 5' | -52.1 | NC_004686.1 | + | 13932 | 0.68 | 0.770025 |
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Target: 5'- ----gGCCGGcgcaaACCGAGGUGCcGUUCGa -3' miRNA: 3'- cauagUGGCU-----UGGCUCUACGaCAGGC- -5' |
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| 19665 | 5' | -52.1 | NC_004686.1 | + | 16646 | 1.09 | 0.002099 |
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Target: 5'- gGUAUCACCGAACCGAGAUGCUGUCCGa -3' miRNA: 3'- -CAUAGUGGCUUGGCUCUACGACAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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