Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 19668 | 3' | -64 | NC_004686.1 | + | 22356 | 1.1 | 0.000139 |
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Target: 5'- cGGCGGGCGGCAGCGGUGACGGCUCCAa -3' miRNA: 3'- -CCGCCCGCCGUCGCCACUGCCGAGGU- -5' |
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| 19668 | 3' | -64 | NC_004686.1 | + | 28786 | 0.66 | 0.297848 |
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Target: 5'- cGGCGGGUGucGCcGCGGUGuuguUGGUguUCCu -3' miRNA: 3'- -CCGCCCGC--CGuCGCCACu---GCCG--AGGu -5' |
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| 19668 | 3' | -64 | NC_004686.1 | + | 28848 | 0.66 | 0.326875 |
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Target: 5'- gGGuuGGCGGCgaAGUGGUcguuGACGGUguucgCCGg -3' miRNA: 3'- -CCgcCCGCCG--UCGCCA----CUGCCGa----GGU- -5' |
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| 19668 | 3' | -64 | NC_004686.1 | + | 36660 | 0.69 | 0.185046 |
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Target: 5'- cGGCGGcGuCGGCGGCGucguaucaacCGGCUCCGg -3' miRNA: 3'- -CCGCC-C-GCCGUCGCcacu------GCCGAGGU- -5' |
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| 19668 | 3' | -64 | NC_004686.1 | + | 37830 | 0.72 | 0.126784 |
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Target: 5'- aGGCGGGCcgucgccccauGCGGCGGgacGACGGC-CCu -3' miRNA: 3'- -CCGCCCGc----------CGUCGCCa--CUGCCGaGGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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