Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 19668 | 3' | -64 | NC_004686.1 | + | 10428 | 0.68 | 0.228416 |
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Target: 5'- gGGCGucguuGGUGGUggugaauucGGCGGUGACGGg-CCGg -3' miRNA: 3'- -CCGC-----CCGCCG---------UCGCCACUGCCgaGGU- -5' |
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| 19668 | 3' | -64 | NC_004686.1 | + | 36660 | 0.69 | 0.185046 |
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Target: 5'- cGGCGGcGuCGGCGGCGucguaucaacCGGCUCCGg -3' miRNA: 3'- -CCGCC-C-GCCGUCGCcacu------GCCGAGGU- -5' |
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| 19668 | 3' | -64 | NC_004686.1 | + | 1025 | 0.7 | 0.160321 |
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Target: 5'- uGGUGGGCGuCGGCGGcUGcCGGUUCgAg -3' miRNA: 3'- -CCGCCCGCcGUCGCC-ACuGCCGAGgU- -5' |
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| 19668 | 3' | -64 | NC_004686.1 | + | 2751 | 0.72 | 0.108163 |
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Target: 5'- uGGUGGGUGGUguggucacAGCGGUGAgGGUcgcCCAa -3' miRNA: 3'- -CCGCCCGCCG--------UCGCCACUgCCGa--GGU- -5' |
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| 19668 | 3' | -64 | NC_004686.1 | + | 16893 | 0.67 | 0.251938 |
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Target: 5'- cGCGGGCacgcugcacgGuGCGGUGGUGGCaGC-CCAg -3' miRNA: 3'- cCGCCCG----------C-CGUCGCCACUGcCGaGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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