Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19675 | 5' | -57.1 | NC_004686.1 | + | 28008 | 0.67 | 0.557698 |
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Target: 5'- --aCCGCUGAacgUGCCauauccacaGCCGCUGaaGCg -3' miRNA: 3'- gaaGGUGACUa--GCGG---------CGGCGACgaCG- -5' |
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| 19675 | 5' | -57.1 | NC_004686.1 | + | 2965 | 0.67 | 0.568432 |
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Target: 5'- --cCUGCUGGU-GUCGgUGCUGCUGUg -3' miRNA: 3'- gaaGGUGACUAgCGGCgGCGACGACG- -5' |
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| 19675 | 5' | -57.1 | NC_004686.1 | + | 39632 | 0.66 | 0.579217 |
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Target: 5'- -aUCUGgUGAUgCGCCGCCGgUGgUGg -3' miRNA: 3'- gaAGGUgACUA-GCGGCGGCgACgACg -5' |
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| 19675 | 5' | -57.1 | NC_004686.1 | + | 21717 | 0.66 | 0.590045 |
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Target: 5'- --gUCACUGGaggaaccccgUCgGUCGCCGUgaagGCUGCg -3' miRNA: 3'- gaaGGUGACU----------AG-CGGCGGCGa---CGACG- -5' |
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| 19675 | 5' | -57.1 | NC_004686.1 | + | 49131 | 0.66 | 0.600907 |
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Target: 5'- aUUCCAUccggGAUUGCaCGUgGCUGUUGa -3' miRNA: 3'- gAAGGUGa---CUAGCG-GCGgCGACGACg -5' |
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| 19675 | 5' | -57.1 | NC_004686.1 | + | 32186 | 0.66 | 0.622702 |
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Target: 5'- -aUCCACcGAgugaacuccaucUUGuuGCaguGCUGCUGCg -3' miRNA: 3'- gaAGGUGaCU------------AGCggCGg--CGACGACG- -5' |
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| 19675 | 5' | -57.1 | NC_004686.1 | + | 49027 | 0.66 | 0.622702 |
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Target: 5'- --aCCACUGAgggGCCGUgGCUGUUc- -3' miRNA: 3'- gaaGGUGACUag-CGGCGgCGACGAcg -5' |
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| 19675 | 5' | -57.1 | NC_004686.1 | + | 31851 | 0.66 | 0.622702 |
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Target: 5'- -cUCCACacGGcgccUUGuCCGCCGCUGCcggugGCg -3' miRNA: 3'- gaAGGUGa-CU----AGC-GGCGGCGACGa----CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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