Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19678 | 5' | -53.8 | NC_004686.1 | + | 39210 | 0.66 | 0.836434 |
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Target: 5'- aCC-CGCCGGcGGCcaggCCCGUGAGg--- -3' miRNA: 3'- -GGuGUGGCCuCUGa---GGGCACUUaguc -5' |
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| 19678 | 5' | -53.8 | NC_004686.1 | + | 39376 | 0.66 | 0.836434 |
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Target: 5'- gCCACGaguUCGGAGACgugCCUuUG-AUCAGg -3' miRNA: 3'- -GGUGU---GGCCUCUGa--GGGcACuUAGUC- -5' |
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| 19678 | 5' | -53.8 | NC_004686.1 | + | 50945 | 0.66 | 0.809024 |
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Target: 5'- gCCGCACCGGuGAgcggguugaUgCCGUGGAugccgaUCAGg -3' miRNA: 3'- -GGUGUGGCCuCUg--------AgGGCACUU------AGUC- -5' |
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| 19678 | 5' | -53.8 | NC_004686.1 | + | 5354 | 0.68 | 0.696579 |
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Target: 5'- aCCugGCUGuuGAGGCUgCUGUGggUCuGg -3' miRNA: 3'- -GGugUGGC--CUCUGAgGGCACuuAGuC- -5' |
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| 19678 | 5' | -53.8 | NC_004686.1 | + | 54945 | 0.69 | 0.663978 |
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Target: 5'- aCGCACCGGAGACgCCCaGU---UCAa -3' miRNA: 3'- gGUGUGGCCUCUGaGGG-CAcuuAGUc -5' |
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| 19678 | 5' | -53.8 | NC_004686.1 | + | 25995 | 0.7 | 0.58734 |
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Target: 5'- aUCACACCcccgaaGGAGAUcCCCGUGAcUUGGa -3' miRNA: 3'- -GGUGUGG------CCUCUGaGGGCACUuAGUC- -5' |
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| 19678 | 5' | -53.8 | NC_004686.1 | + | 4179 | 0.71 | 0.55487 |
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Target: 5'- uCCACGCCGGaAGAUcgugCCCGcgugcggcaaUGGAUCAa -3' miRNA: 3'- -GGUGUGGCC-UCUGa---GGGC----------ACUUAGUc -5' |
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| 19678 | 5' | -53.8 | NC_004686.1 | + | 33366 | 0.74 | 0.377003 |
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Target: 5'- gCgACACUGGAGGCUCCUGuUGAGccugCAGc -3' miRNA: 3'- -GgUGUGGCCUCUGAGGGC-ACUUa---GUC- -5' |
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| 19678 | 5' | -53.8 | NC_004686.1 | + | 5280 | 0.75 | 0.318134 |
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Target: 5'- aCACGCCGGAcaaacagGGCUCUCGUGGAUgAa -3' miRNA: 3'- gGUGUGGCCU-------CUGAGGGCACUUAgUc -5' |
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| 19678 | 5' | -53.8 | NC_004686.1 | + | 26152 | 1.1 | 0.001369 |
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Target: 5'- aCCACACCGGAGACUCCCGUGAAUCAGc -3' miRNA: 3'- -GGUGUGGCCUCUGAGGGCACUUAGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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