Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19679 | 5' | -56.9 | NC_004686.1 | + | 22722 | 0.66 | 0.652132 |
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Target: 5'- aGCGCa-GCCAGGGAcggaguUCGUccuuugCCGUGg -3' miRNA: 3'- gUGCGgcCGGUCCCU------AGUAua----GGCAC- -5' |
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| 19679 | 5' | -56.9 | NC_004686.1 | + | 41479 | 0.66 | 0.637992 |
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Target: 5'- -uUGuuGGCgGGGGAgcggaaagccgccgUCGUAUCCGg- -3' miRNA: 3'- guGCggCCGgUCCCU--------------AGUAUAGGCac -5' |
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| 19679 | 5' | -56.9 | NC_004686.1 | + | 49973 | 0.66 | 0.616228 |
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Target: 5'- gGCGCCGuaCGGGGAgucacuguggacguUCGUGUgCCGg- -3' miRNA: 3'- gUGCGGCcgGUCCCU--------------AGUAUA-GGCac -5' |
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| 19679 | 5' | -56.9 | NC_004686.1 | + | 3842 | 0.67 | 0.565419 |
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Target: 5'- aCGCGCUGGCCGGGuGGguggCGgaaCCGUu -3' miRNA: 3'- -GUGCGGCCGGUCC-CUa---GUauaGGCAc -5' |
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| 19679 | 5' | -56.9 | NC_004686.1 | + | 2009 | 0.67 | 0.544103 |
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Target: 5'- uCGCGCCGGCa--GGAUCGUAcUUCGg- -3' miRNA: 3'- -GUGCGGCCGgucCCUAGUAU-AGGCac -5' |
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| 19679 | 5' | -56.9 | NC_004686.1 | + | 47990 | 0.67 | 0.544103 |
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Target: 5'- gACGCCGGCCGGGu-UC---UCCGg- -3' miRNA: 3'- gUGCGGCCGGUCCcuAGuauAGGCac -5' |
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| 19679 | 5' | -56.9 | NC_004686.1 | + | 400 | 0.68 | 0.523056 |
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Target: 5'- uUACGCCGacGCCGGGcGGUCAaccggcggGUCCGa- -3' miRNA: 3'- -GUGCGGC--CGGUCC-CUAGUa-------UAGGCac -5' |
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| 19679 | 5' | -56.9 | NC_004686.1 | + | 27457 | 0.68 | 0.502329 |
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Target: 5'- cCGCGCCGcGCCAaGGA-CGcUAUCUGUGc -3' miRNA: 3'- -GUGCGGC-CGGUcCCUaGU-AUAGGCAC- -5' |
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| 19679 | 5' | -56.9 | NC_004686.1 | + | 26282 | 1.08 | 0.000773 |
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Target: 5'- gCACGCCGGCCAGGGAUCAUAUCCGUGg -3' miRNA: 3'- -GUGCGGCCGGUCCCUAGUAUAGGCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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