Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 19681 | 3' | -57.7 | NC_004686.1 | + | 50108 | 0.66 | 0.616943 |
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Target: 5'- gGGUCGGCGGccaGGCaUGGCGcgaccCGUGAc -3' miRNA: 3'- -UCAGCCGCUuc-CCGaGCCGUa----GCACU- -5' |
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| 19681 | 3' | -57.7 | NC_004686.1 | + | 26736 | 0.66 | 0.606151 |
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Target: 5'- -aUCGGCGAGaaaCUCGGCAUCGa-- -3' miRNA: 3'- ucAGCCGCUUcccGAGCCGUAGCacu -5' |
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| 19681 | 3' | -57.7 | NC_004686.1 | + | 9741 | 0.66 | 0.584642 |
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Target: 5'- aAGUCGGUGAAGGGCaCcGCuuUCGg-- -3' miRNA: 3'- -UCAGCCGCUUCCCGaGcCGu-AGCacu -5' |
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| 19681 | 3' | -57.7 | NC_004686.1 | + | 47519 | 0.66 | 0.563285 |
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Target: 5'- -uUCGGCGGAGcGGga-GGC-UCGUGAg -3' miRNA: 3'- ucAGCCGCUUC-CCgagCCGuAGCACU- -5' |
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| 19681 | 3' | -57.7 | NC_004686.1 | + | 18886 | 0.68 | 0.490572 |
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Target: 5'- aAGUCGGCGguGGuacGUUCGGCGaaggugcCGUGGa -3' miRNA: 3'- -UCAGCCGCuuCC---CGAGCCGUa------GCACU- -5' |
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| 19681 | 3' | -57.7 | NC_004686.1 | + | 36872 | 0.7 | 0.368082 |
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Target: 5'- gAGuUCGGCG-AGGGCUgcucauaaggugaUGGCGUCG-GAu -3' miRNA: 3'- -UC-AGCCGCuUCCCGA-------------GCCGUAGCaCU- -5' |
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| 19681 | 3' | -57.7 | NC_004686.1 | + | 4370 | 0.71 | 0.297693 |
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Target: 5'- gAGUUGGCGGGugguGGGUUUGGgGUCGaUGAg -3' miRNA: 3'- -UCAGCCGCUU----CCCGAGCCgUAGC-ACU- -5' |
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| 19681 | 3' | -57.7 | NC_004686.1 | + | 21394 | 0.75 | 0.187991 |
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Target: 5'- uGGgggCGGUGucuGGuGCUCGGCGUCGUGc -3' miRNA: 3'- -UCa--GCCGCuu-CC-CGAGCCGUAGCACu -5' |
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| 19681 | 3' | -57.7 | NC_004686.1 | + | 40520 | 0.76 | 0.139925 |
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Target: 5'- aGGUC-GCGGAGGGa--GGCGUCGUGAu -3' miRNA: 3'- -UCAGcCGCUUCCCgagCCGUAGCACU- -5' |
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| 19681 | 3' | -57.7 | NC_004686.1 | + | 27731 | 1.08 | 0.000677 |
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Target: 5'- cAGUCGGCGAAGGGCUCGGCAUCGUGAu -3' miRNA: 3'- -UCAGCCGCUUCCCGAGCCGUAGCACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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